Patho-physiological studies on the Reverse Effect of Curcumin (Curcuma longa, Zingiberaceae) and Ursofalk (Ursodeoxycholic acid) against the Toxicity of Carbon Tetrachloride on Albino Rats

Abstract

This study, was established five groups of albino rats to determine the therapeutic effect of Curcuma longa and ursofalk against the toxicity of CC14 in liver and kidney. Group (1) was received orally NaCl 0.9% and used as a normal group. Group (2) was injected intraperitoneal (i.p.) with CCl4 (1 ml/kg), 3 times weekly, for 2 weeks. Group (3) was given orally Ursofalk (100 mg/kg per body weight), group (4) was given orally Curcuma longa (100 mg/kg body weight) and group (5) was given the same doses of Ursofalk plus Curcuma longa for 30 days respectively, post-injected intraperitoneal (i.p.) with CCl4 (1 ml/kg) 3 times weekly, for 2 weeks. Two blood samples were collected, for hematological and biochemical parameters. Specimens from liver and kidney were collected for histopathological examination. Group (2) revealed a highly significant decrease in total RBCs, platelets, Hb and PCV, serum uric acid, albumin, glucose, HDL-cholesterol, besides catalase, GSH, SOD activities in liver tissue, while WBCs, serum ALT, AST, ALP, γ-GT, creatinine, urea, cholesterol, triglycerides and LDL-cholesterol levels, besides Malondialdehyde and Nitric Oxide levels in liver tissue showed a highly significant increase. Meanwhile, groups (3, 4 and 5) displayed reverse effect in all parameters and return to normal. The histological results displayed inflammation with necrosis and degenerative changes in group (2), while remain groups showed mild changes particularly in group (5). It could be concluded that CCl4 induced destruction in liver and kidney, which showed a clear improvement by using of Ursofalk and Curcuma longa as treatment.