Feasibility study of atmospheric pressure plasma treatments of HEPG-2 and SK-HEP-1 cancer cells

B. Gweon, D. Kim, S. Moon, W. Choe, Sukhyun Song, Daeyun Kim, J. Shin
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Abstract

Summary form only given. A feasibility study of plasma cancer cell treatment was performed using a pin-type plasma jet. A 50 kHz AC power was used to generate the plasma in the ambient air assisted by the helium flow. The plasma gas temperature was maintained at the room temperature of 25degC to eliminate the thermal damages to the cell. The liver cancer cells of HEPG-2 and SK-HEP-1 were used as the treatment samples. The sample cells were prepared on the fibronectin coated slide glass for the sake of convenience of the microscopic observation process. The input voltage and the treatment time were controlled in the range of (750-1000) V and (30-120) s, respectively. After the plasma treatments, the sample cells were stained with the viability assay (ethidium homodimer and calcein AM) to make discrimination between live and dead cells. Then, the microscopy scan was performed from the plasma center up to about 1 cm. The necrotized cells as well as a void or cell free zone appeared starting at the input voltage of 800 V and the treatment time of 60 s, . The necrotized zone and the void were well matched to the plasma profile. Normal liver cell, THLE-2, was then treated for comparison with the liver cancer cells.
常压等离子体治疗HEPG-2和SK-HEP-1癌细胞的可行性研究
只提供摘要形式。采用针型等离子体射流进行了治疗等离子体癌细胞的可行性研究。在氦气流的辅助下,使用50khz交流电源在周围空气中产生等离子体。等离子体气体温度保持在室温25℃,以消除对电池的热损伤。以肝癌细胞HEPG-2和SK-HEP-1为治疗样本。为了便于显微镜观察,将样品细胞制备在纤维连接蛋白包被玻片上。输入电压控制在(750 ~ 1000)V,处理时间控制在(30 ~ 120)s。血浆处理后,对样品细胞进行活力染色(乙啶同型二聚体和钙黄蛋白AM),区分活细胞和死细胞。然后,从等离子体中心到大约1cm处进行显微镜扫描。在输入电压为800 V,处理时间为60 s时,开始出现坏死细胞和空洞或无细胞区。坏死区和空洞与等离子体轮廓吻合得很好。然后对正常肝细胞THLE-2进行处理,与肝癌细胞进行比较。
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