Antitumor efficacy of Urtica sp. leaves extract: in vitro and in vivo studies

Journal of Bioscience and Applied Research Pub Date : 2022-11-07 DOI:10.21608/jbaar.2022.268775

Rania Fakher El Deen, S. El-Naggar, E. El-Nahass, K. El-Said

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引用次数: 1

Abstract

Background: Natural products demonstrated potential for use in medicine and pharmaceuticals. Objective: This study evaluated the phytochemical composition of Urtica sp. leaves extract (USLE), and it’s in vitro and in vivo antitumor effects. Methods: HepG-2 and MCF-7 cell lines were used to evaluate the IC 50 , apoptosis, and cell cycle analysis of USLE. Forty female CD-1 mice were equally divided as follows; Gp1 was control; Gp2, Gp3, and Gp4 were inoculated with 1 × 10 6 of Ehrlich ascitic carcinoma (EAC)-cells/mouse. Then, Gp3 had injected with Cisplatin (2 mg/kg) intraperitoneally (i.p). Gp4 had injected with USLE (100 mg/kg) i.p. as in Gp3. Total tumor volume, total tumor cell count, and live and dead EAC-cells were determined, also biochemical and histopathological investigations were evaluated. Results: Data showed that the USLE had cytotoxic and antitumor effects against the HepG-2, MCF-7, and EAC-bearing mice. Biochemical and histopathological investigations showed an improvement in the liver and kidney tissues upon treatment of EAC-bearing mice with USLE. Conclusion: The results showed that the USLE can stop tumor growth and cause tumor cells to die.

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荨麻叶提取物的抗肿瘤作用:体外和体内研究

背景:天然产物在医学和药品中具有潜在的用途。目的:研究荨麻叶提取物(USLE)的植物化学成分及其体外、体内抗肿瘤作用。方法:采用HepG-2和MCF-7细胞系对USLE进行ic50、凋亡和细胞周期分析。40只雌性CD-1小鼠平均分组如下:Gp1为对照;Gp2、Gp3和Gp4分别接种1 × 106个Ehrlich腹水癌细胞/小鼠。然后给Gp3腹腔注射顺铂(2 mg/kg)。Gp4注射USLE (100 mg/kg)，与Gp3相同。测定肿瘤总体积、肿瘤总细胞数、活细胞和死细胞，并进行生化和组织病理学检查。结果:USLE对HepG-2、MCF-7、eac小鼠均有细胞毒和抗肿瘤作用。生化和组织病理学检查显示，用USLE治疗eac小鼠后，肝脏和肾脏组织有改善。结论:USLE具有抑制肿瘤生长、致肿瘤细胞死亡的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Journal of Bioscience and Applied Research

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