Sub-inhibitory doses of Ofloxacin reduce adhesion and biofilm formation of Pseudomonas aeruginosa to biotic and abiotic surfaces

Q4 Pharmacology, Toxicology and Pharmaceutics

Pharmaceutical Sciences Asia Pub Date : 2023-01-01 DOI:10.29090/psa.2023.03.23.377

Lubna Ali Abd Al-mutalib, A. Zgair

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Abstract

Biofilm formation of Pseudomonas aeruginosa ( P. aeruginosa ) and its adherence increases bacterial virulence to cause the infection. The role of treating P. aeruginosa with sub-inhibitory concentrations of ofloxacin in vitro in reducing bacterial adherence to biotic and abiotic surfaces was evaluated. Ten isolates of P. aeruginosa were isolated from urine samples. Biofilm formation on polystyrene microtiter plates and Minimum inhibitory concentrations (MICs) of ofloxacin against all isolates were evaluated. The effect of sub-MICs of ofloxacin (0.5×MIC, 0.25×MIC, 0.125×MIC, and 0.06×MIX) on biofilm formation (to polystyrene) and adhesion to prepared human epithelial cells (HECs) in vitro was evaluated. The MICs of ofloxacin were lower than 64 µg/ml and all isolates produced biofilm. There was no relationship between the susceptibility of bacterial isolates to ofloxacin and biofilm formation (r:-0.11; P >0.05). It was found that all sub-MIC concentrations of ofloxacin reduced significantly the biofilm formation on polystyrene and adhesion to HECs in a concentration-dependent manner. Electron microscope images showed that the sub-MIC concentrations of ofloxacin prevent biofilm development to maturation. It can be concluded the negative effect of sub-MIC concentrations of ofloxacin on biofilm production and adhesion to biotic and abiotic surfaces in vitro .

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氧氟沙星亚抑制剂量降低铜绿假单胞菌对生物和非生物表面的粘附和生物膜形成

铜绿假单胞菌(P. aeruginosa)的生物膜形成及其粘附性增加了细菌的毒力，从而引起感染。用亚抑制浓度的氧氟沙星治疗铜绿假单胞菌在体外减少细菌对生物和非生物表面的粘附作用进行了评估。从尿样中分离到铜绿假单胞菌10株。测定了聚苯乙烯微滴板上的生物膜形成情况和氧氟沙星对所有分离株的最低抑菌浓度(mic)。研究了氧氟沙星亚mic (0.5×MIC, 0.25×MIC, 0.125×MIC和0.06×MIX)对体外制备的人上皮细胞(hec)的生物膜形成(对聚苯乙烯)和粘附的影响。氧氟沙星的mic均低于64µg/ml，所有分离株均产生生物膜。分离细菌对氧氟沙星的敏感性与生物膜的形成没有关系(r:-0.11;P > 0.05)。结果发现，所有亚mic浓度的氧氟沙星都能显著降低聚苯乙烯上生物膜的形成和对HECs的粘附，并呈浓度依赖性。电镜图像显示，亚mic浓度的氧氟沙星阻止生物膜发育成熟。由此可见，亚mic浓度的氧氟沙星对体外生物膜的生成及与生物和非生物表面的粘附均有负面影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pharmaceutical Sciences Asia Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)

CiteScore

0.90

自引率

0.00%

发文量

期刊介绍： The Pharmaceutical Sciences Asia (PSA) journal is a double-blinded peer-reviewed journal in English published quarterly, by the Faculty of Pharmacy, Mahidol University, Thailand. The PSA journal is formerly known as Mahidol University Journal of Pharmaceutical Sciences and committed to the timely publication of innovative articles and reviews. This journal is available in both printed and electronic formats. The PSA journal aims at establishing a publishing house that is open to all. It aims to disseminate knowledge; provide a learned reference in the field; and establish channels of communication between academic and research expert, policy makers and executives in industry and investment institutions. The journal publishes research articles, review articles, and scientific commentaries on all aspects of the pharmaceutical sciences and multidisciplinary field in health professions and medicine. More specifically, the journal publishes research on all areas of pharmaceutical sciences and related disciplines: Clinical Pharmacy Drug Synthesis and Discovery Targeted-Drug Delivery Pharmaceutics Biopharmaceutical Sciences Phytopharmaceutical Sciences Pharmacology and Toxicology Pharmaceutical Chemistry Nutraceuticals and Functional Foods Natural Products Social, Economic, and Administrative Pharmacy Clinical Drug Evaluation and Drug Policy Making Antimicrobials, Resistance and Infection Control Pharmacokinetics and Pharmacodynamics.