Rhodamine B Temperature Dosimetry of Biological Samples Interacting with Electromagnetic Fields in Macrosystems

2019 IEEE MTT-S International Microwave Symposium (IMS) Pub Date : 2019-06-02 DOI:10.1109/mwsym.2019.8700784

A. Nefzi, Lynn Carr, C. Dalmay, A. Pothier, P. Lévêque, D. Arnaud-Cormos

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Abstract

Exposing living cells to a certain level of Electromagnetic Field (EMF) might induce some biological effects including temperature elevation. In this paper, we show the dosimetry of exposure systems such as an Open Transverse Electro-Magnetic (TEM) cell allowing the study of the effect of EMF on biological samples exposed to 1.8 GHz signals. Temperature measurements are carried out with a fluorooptic probe to extract specific absorption rate (SAR) values that are compared to numerical dosimetry, based on a FDTD method. To investigate dosimetry at a microscopic level the fluorescence of the temperature dependent dye Rhodamine B was measured with fluorescence microscopy. The results are confirmed by measurements and simulations with a SAR of 13.9 and 11.8 W/kg for 1 W incident power, respectively. Results evidence that the objective working distance of the microscope strongly influence SAR values. After calibration, the fluorescence fits well with the temperature variation measured by the probe.

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大系统中生物样品与电磁场相互作用的罗丹明B温度剂量测定

将活细胞暴露于一定水平的电磁场(EMF)中可能会引起一些生物效应，包括温度升高。在本文中，我们展示了暴露系统的剂量学，例如开放式横向电磁(TEM)细胞，允许研究EMF对暴露于1.8 GHz信号的生物样品的影响。温度测量采用荧光光学探针提取特定吸收率(SAR)值，并与基于时域有限差分法的数值剂量法进行比较。为了在显微水平上研究剂量学，用荧光显微镜测量了温度依赖性染料罗丹明B的荧光。实验结果表明，当入射功率为1w时，SAR分别为13.9 W/kg和11.8 W/kg。结果表明，显微镜物镜工作距离对SAR值影响较大。校正后，荧光与探针测得的温度变化吻合良好。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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2019 IEEE MTT-S International Microwave Symposium (IMS)

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