009 Skin aging and photoaging effects can be quantified in vivo by fluoresence excitation spectroscopy

N. Kolias, G. Stamatas
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引用次数: 3

Abstract

Fluoresence excitation spectroscopy of skin is a noninvasive method for objective evaluations of skin aging and photoaging. Fluoresence bands in the UV have been assigned to tryptophan moieties, pepsin digestible collagen cross-links (PDCCL), collagenase digestible collagen cross-links (CDCCL) and elastin cross-links. We have studied the dependence of these bands to aging and photoaging in the hairless mouse model and in humans. In the mouse, gluorescence of the tryptophan moieties decreases linearly with age, while the fluorescence band due to PDCCL increases linearly with age. In contrast, chronically UVB exposed mice showed an increase in the tryptophan band and a dramatic reduction of the PDCCL band compared to age-matched controls. The same phenomena were observed on mouse skin immediately after UVA exposure. The dependence of fluorescence of human facial skin with aging was studied in a large population sample (> 500 people in total) ages 15–70 at five geographic locations. Similar to the mouse model we observed a decrease in the tryptophan fluorescence signal, which is probably related to the reduction of the cell turnover rate with aging. Furthermore, observed increases in the fluorescence signals corresponding to collagen and elastin cross-links with aging may be attributed to chronic accumulation of cross-links in these long-lived molecules. The fluorescence ratio of the elastin cross-links signal to that of tryptophan moieties correlates strongly with age and is independent of geographical region and seasonal effects. The same fluorescence ratio has been used to monitor the antiaging effects of retinol treatment.
009皮肤老化和光老化效应可通过荧光激发光谱学在体内量化
皮肤荧光激发光谱是客观评价皮肤老化和光老化的一种无创方法。紫外荧光带被分配到色氨酸部分,胃蛋白酶可消化胶原交联(PDCCL),胶原酶可消化胶原交联(CDCCL)和弹性蛋白交联。我们在无毛小鼠模型和人类中研究了这些条带对衰老和光老化的依赖性。在小鼠中,色氨酸部分的荧光随着年龄的增长而线性下降,而PDCCL引起的荧光带随着年龄的增长而线性增加。相比之下,与年龄匹配的对照组相比,长期暴露于UVB的小鼠表现出色氨酸带的增加和PDCCL带的急剧减少。在UVA暴露后立即在小鼠皮肤上观察到同样的现象。在5个地理位置的15-70岁的大样本人群(约500人)中,研究了面部皮肤荧光与衰老的相关性。与小鼠模型相似,我们观察到色氨酸荧光信号的减少,这可能与细胞周转率随着年龄的增长而降低有关。此外,观察到的与胶原蛋白和弹性蛋白交联相关的荧光信号随衰老的增加可能归因于这些长寿命分子中交联的慢性积累。弹性蛋白交联信号与色氨酸部分的荧光比与年龄密切相关,不受地理区域和季节的影响。采用相同的荧光比监测视黄醇治疗的抗衰老效果。
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