In vitro cytotoxic effects of different intracanal medicaments on the co-culture of dental mesenchymal stem cells using the MTT assay

IF 0.5 Q4 DENTISTRY, ORAL SURGERY & MEDICINE

Giornale Italiano di Endodonzia Pub Date : 2021-04-07 DOI:10.32067/GIE.2021.35.01.13

E. Saberi, Farshid Gholami, Arezoo Pirhaji, Elahe Shahreki

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引用次数: 1

Abstract

Aim: This in vitro study aimed to assess the cytotoxic effects of different intracanal medicaments on the co-culture of dental mesenchymal stem cells. Materials and Methods: The Periodontal Ligament Stem Cells (PDLSCs) and the stem cells of the apical papilla (SCAPs) were isolated from a human teeth. The cells were passaged and underwent mono-culture and co-culture. The cells were then exposed to different concentrations of calcium hydroxide/chlorhexidine, calcium hydroxide/distilled water, double antibiotic paste/ chlorhexidine, and double antibiotic paste/distilled water. The cytotoxicity and cell viability was evaluated and data were analyzed by independent t-test, ANOVA, and Tukey’s test (alpha=0.05). Results: The viability of PDLSCs in co-culture was higher than that in mono-culture following exposure to different concentrations of medicaments. However, when distilled water was used as the vehicle for intracanal medicaments, the viability of SCAPs in mono-culture was higher than that in co-culture. In use of DAP/CHX, the viability of SCAP in co-culture was higher than that in mono-culture (P=0.000). This difference was not significant in exposure of SCAP to DAP/CHX (P>0.05). Conclusions: Generally, the viability of PDLSCs in co-culture was higher than that in mono-culture, and distilled water was a better vehicle than CHX for intracanal medicaments.

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用MTT法观察不同管内药物对牙间充质干细胞共培养的体外细胞毒作用

目的:探讨不同药物对牙间充质干细胞共培养的细胞毒性作用。材料与方法:从人牙齿中分离得到牙周韧带干细胞(PDLSCs)和根尖乳头干细胞(SCAPs)。细胞传代，分别进行单培养和共培养。然后将细胞暴露于不同浓度的氢氧化钙/氯己定、氢氧化钙/蒸馏水、双抗生素膏/氯己定和双抗生素膏/蒸馏水中。评估细胞毒性和细胞活力，采用独立t检验、方差分析和Tukey检验(α =0.05)分析数据。结果:不同浓度药物作用后，共培养的PDLSCs的存活率均高于单培养。然而，当蒸馏水作为载药剂时，单培养的SCAPs活力高于共培养。在使用DAP/CHX时，共培养的SCAP活力高于单培养(P=0.000)。SCAP暴露于DAP/CHX时，差异不显著(P < 0.05)。结论:一般情况下，共培养的PDLSCs的存活率高于单培养，蒸馏水比CHX更适合用于管内给药。

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来源期刊

Giornale Italiano di Endodonzia DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

0.90

自引率

25.00%

发文量

审稿时长

24 weeks

期刊介绍： The Giornale Italiano di Endodonzia was founded in 1987 and is the official journal of the Italian Society of Endodontics (SIE). It is a peer-reviewed journal publishing original articles on clinical research and/or clinical methodology, case reports related to Endodontics. The Journal evaluates also contributes in restorative dentistry, dental traumatology, experimental pathophysiology, pharmacology and microbiology dealing with Endodontics.