Comparative Evaluation of the Effectiveness of a Combination of Absorbable Gelatin Sponge and Calendula officinalis with Absorbable Gelatin Sponge Used Alone as a Hemostatic Agent—An In-Vitro Study

IF 0.5 Q4 DENTISTRY, ORAL SURGERY & MEDICINE
Bharath Kumar Ayyanahalli Matta, Santhosh Kumar, C. Mehta, U. Nayak, Patricia Rodríguez
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引用次数: 2

Abstract

Excessive bleeding can complicate surgical intervention; this could be managed using an effective hemostatic agent that provides immediate and early bleeding control. Gelatin sponge and Calendula officinalis have been proven to have good hemostatic properties. The present In-vitro study analyzed the cytotoxicity and hemostatic properties of gelatin sponge and Calendula officinalis. The cytotoxic concentration/effective concentration of Calendula officinalis was determined by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay. The drug release was determined using a vertical Franz diffusion cell apparatus; solid-state characterization was assessed using Fourier-transform infrared spectroscopy (FTIR) and a differential scanning calorimeter (DSC). The MTT assay showed 7% Calendula officinalis to be cytocompatible, and there was an increase in cell proliferation. When the 7% Calendula officinalis was loaded into the sponge, it was compatible, and the drug content was found to be 56.28 ± 13.84%. The time taken for the blood clot formation was measured using the Lee–White method. The gelatin sponge’s time for clot formation was 161.70 ± 3.11 s, and the Calendula officinalis loaded gelatin sponge’s time for clot formation was 158.75 ± 4.60 s. Hence, it could be concluded that when Calendula officinalis is incorporated into a gelatin sponge, it shows material compatibility and cytocompatibility, reduces the time for clot formation, and could be used as an alternative to other hemostatic agents.
可吸收性明胶海绵与金盏花联合使用与单独使用可吸收性明胶海绵止血效果的体外比较研究
出血过多会使手术干预复杂化;这可以使用一种有效的止血剂来管理,这种止血剂可以提供立即和早期的出血控制。明胶海绵和金盏花已被证明具有良好的止血性能。本实验分析了明胶海绵和金盏花的细胞毒性和止血性能。采用MTT(3-[4,5-二甲基噻唑-2-基]-2,5二苯基溴化四唑)法测定金盏菊的细胞毒浓度/有效浓度。采用垂直Franz扩散池法测定药物释放度;使用傅里叶变换红外光谱(FTIR)和差示扫描量热计(DSC)评估固态表征。MTT试验显示,7%金盏菊具有细胞相容性,细胞增殖能力增强。将7%金盏菊装入海绵后,与海绵配伍,药物含量为56.28±13.84%。用Lee-White法测量血凝块形成所需的时间。明胶海绵的凝块形成时间为161.70±3.11 s,金盏花负载明胶海绵的凝块形成时间为158.75±4.60 s。由此可见,金盏菊加入明胶海绵后,具有良好的材料相容性和细胞相容性,可缩短凝块形成时间,可作为其他止血药物的替代品。
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来源期刊
Open Dentistry Journal
Open Dentistry Journal DENTISTRY, ORAL SURGERY & MEDICINE-
CiteScore
2.00
自引率
0.00%
发文量
86
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