Effect of oxygen tension on proliferation and characteristics of Wharton's jelly-derived mesenchymal stem cells

Biomarkers and Genomic Medicine Pub Date : 2014-03-01 DOI:10.1016/j.bgm.2014.02.001

Wahyu Widowati , Laura Wijaya , Indra Bachtiar , Rimonta F. Gunanegara , Sri Utami Sugeng , Yudha Aryadi Irawan , Sutiman B. Sumitro , M. Aris Widodo

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引用次数: 32

Abstract

Mesenchymal stem cells (MSCs) from Wharton's jelly have a higher proliferation rate and self-renewal capacity than adult tissue-derived MSCs. A low oxygen level or hypoxic condition is prevalent in the microenvironment of the stem cells in the early stages of development. Hypoxia can influence proliferation and differentiation of various stem/precursor cell populations. This research was conducted: to determine the proliferation rate and characteristics of human MSCs from Wharton's jelly in hypoxic and normoxic condition; to evaluate their character after MSCs are incubated in hypoxic and normoxic environment using surface markers including CD105, CD73, CD14, CD19, CD34, CD45, and HLA-II; and to evaluate the proliferation rate and number of MSCs at many passages using the trypan blue method. The hypoxic and normoxic microenvironment showed significant differences in the proliferation rate and population doubling time, but and there were no differences in surface markers.

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氧张力对Wharton氏胶状间充质干细胞增殖及特性的影响

华氏水母间充质干细胞(MSCs)比成体组织来源的间充质干细胞具有更高的增殖率和自我更新能力。低氧水平或缺氧条件在干细胞发育早期的微环境中普遍存在。缺氧可以影响各种干细胞/前体细胞群的增殖和分化。本研究旨在研究缺氧和常氧条件下华氏水母人间充质干细胞的增殖速率和特性;利用CD105、CD73、CD14、CD19、CD34、CD45和HLA-II等表面标记物评价MSCs在缺氧和常氧环境下培养后的特性;台盼蓝法测定多传代MSCs的增殖率和数量。低氧微环境和常氧微环境在增殖速率和群体倍增时间上存在显著差异，但在表面标记物上无显著差异。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Biomarkers and Genomic Medicine

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