HPTLC AND GC-MS Analysis of Methanolic Extracts of Samanea Saman (Albizia Saman) (Jacquin) F. Mueller

S. Shanmugakumar, G. Kumar, K. Padmalatha
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Abstract

Samanea saman (Albizia saman) is a wide-canopied tree with a large symmetrical crown. It usually reaches a height of 25 m (82 ft) and a diameter of 40 m. The leaves fold in rainy weather and in the evening, hence the name "rain tree" and "five o'clock tree" (Pukul Lima) in Malay. In the present investigation, the bioactive methanolic fraction was subjected to HPTLC and GC-MS analysis. GCMS analysis revealed the presence of six compounds from the methanolic fraction of the Samanea saman (1) 9,Octadecenoic acid (Z) methyl ester, (2) Dodecanoic acid, 10-methyl, methyl ester, (3) 13Hexyloxyacylotridec-10-en-2-one, (4) 15Octadecenoic acid, methyl ester (5) Diethyl -1(8 –amino -1naphthyl) -1,2,3 triazole -4,5 –dicarboxylate were identified from the methanolic extract from the leaves of Samanea saman. The presence of these compounds was confirmed by NIST library values. HPTLC analysis concludes the presence of four major phytoconstituents which was eluted at Rf 0.67; 0.79; 0.85 & 0.90 respectively.
花楸楸(Albizia Saman) (Jacquin)甲醇提取物的hplc - GC-MS分析
沙曼树(Albizia saman)是一种树冠宽大、树冠对称的树。它通常高达25米(82英尺),直径40米。在雨天和晚上,叶子会折叠,因此在马来语中被称为“雨树”和“五点钟树”(Pukul Lima)。在本研究中,对生物活性甲醇部分进行了HPTLC和GC-MS分析。从沙蔓叶甲醇提取物中鉴定出6个化合物(1)9,十八烯酸(Z)甲酯,(2)十二烷酸,10-甲基,甲酯,(3)13 -己氧酰基三烯-10-烯-2 -1,(4)15十八烯酸甲酯,(5)二乙基-1(8 -氨基-1萘基)-1,2,3三唑-4,5 -二羧酸酯。这些化合物的存在被NIST文库值证实。HPTLC分析结果表明,样品中存在4种主要植物成分,洗脱Rf为0.67;0.79;分别为0.85和0.90。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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