Effects of fumonisin B1 on the immune system of sprague-dawley rats following a 14-day oral (gavage) exposure.

H. Tryphonas, G. Bondy, J. Miller, F. Lacroix, M. Hodgen, P. Mcguire, S. Fernie, D. Miller, S. Hayward
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引用次数: 37

Abstract

The effects of fumonisin B1 (FB1) on the immune system of Sprague-Dawley rats were investigated. Groups of male and female rats (10 rats/group) were gavaged daily for 14 days with doses of 0, 5, 15, and 25 mg/kg body wt/day and the primary (IgM) response to sheep red blood cells expressed as plaque-forming cell numbers/10(6) spleen mononuclear leukocytes (PFC/10(6) splenocytes) and PFC/spleen was determined. There was a significant dose-related linear trend toward decreased PFC/10(6) splenocytes (p = 0.003) and PFC/spleen cells (p = 0.001) in the male rats. Body weights, expressed as a percentage of the control, were significantly reduced (p = 0.002) in the male rats administered 15 and 25 mg/kg doses. The PFC numbers in female rats were not affected significantly by treatment (p > 0.05). For the remaining immunotoxicity studies, groups of male rats (10 rats/group) were gavaged with FB1 doses of 0, 1, 5, and 15 mg/kg body wt/day for 14 days. There was a weakly significant dose-related trend toward increased numbers of serum immunoglobulin class G (p = 0.04). Also a significant dose-related increase (p = 0.013) in Listeria monocytogenes numbers was observed in the spleen at 24 hr postinfection. Treatment did not have a significant effect on organ weights, hematology, mitogen-induced lymphocyte transformation, calcium mobilization, the numbers of leukocytes and T-lymphocyte subsets, the natural killer cell activity, and phagocytosis (p >/= 0. 05). These observations suggested that FB1 may have indirect consequences for human health and warrant further investigations.
伏马菌素B1对sprague-dawley大鼠14天后免疫系统的影响
研究伏马菌素B1 (FB1)对Sprague-Dawley大鼠免疫系统的影响。以0、5、15和25 mg/kg体重量/天的剂量,每天灌胃雄性和雌性大鼠组(10只/组),连续14天,测定绵羊红细胞对血小板形成细胞数/10(6)个脾脏单核白细胞(PFC/10(6)个脾细胞)和PFC/脾脏的主要(IgM)反应。雄性大鼠PFC/10(6)脾细胞和PFC/脾细胞呈显著的剂量相关线性趋势(p = 0.003)。在15和25 mg/kg剂量的雄性大鼠中,体重(以对照的百分比表示)显著降低(p = 0.002)。治疗对雌性大鼠PFC数无显著影响(p < 0.05)。在其余免疫毒性研究中,雄性大鼠组(10只/组)分别以0、1、5和15 mg/kg体重量/天的剂量灌胃FB1,持续14天。血清免疫球蛋白G类增加呈弱显著剂量相关趋势(p = 0.04)。在感染后24小时,脾脏中单核增生李斯特菌数量也有显著的剂量相关增加(p = 0.013)。治疗对器官重量、血液学、丝裂原诱导的淋巴细胞转化、钙动员、白细胞和t淋巴细胞亚群的数量、自然杀伤细胞活性和吞噬能力没有显著影响(p >/= 0)。05). 这些观察结果表明,FB1可能对人类健康产生间接影响,值得进一步调查。
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