Identification of four Indian ascidians based on COI gene sequences

IF 1.1 4区生物学 Q4 GENETICS & HEREDITY

Mitochondrial Dna Part a Pub Date : 2018-01-02 DOI:10.1080/24701394.2016.1233531

H. A. Jaffarali, Soban A Akram, Kaleem M L Arshan

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引用次数: 6

Abstract

Abstract DNA barcoding involving the sequencing of a short mitochondrial DNA segment, cytochrome c oxidase subunit 1 (COI) gene, is a specialized technique for the identification of species even at the early embryonic and larval stages, which is quite difficult in morphology-based taxonomy. Ascidians are sessile invertebrate chordates possessing numerous biochemical as well as pharmacological activities. In this study, a total of 36 ascidian samples belonging to the family Didemnidae were sequenced for a 650 bp region of the mitochondrial COI gene. All the species were represented by multiple specimens. The barcode sequences showed no stop-codons and indels in the alignments. The aligned sequences were submitted in Barcode submission tool, NCBI, and the accession numbers were obtained. The minimum intraspecific distance was found to be 0.00% and the maximum was 2.23%. Mean Kimura 2-parameter (K2P) distances within-species, genus, and family were 0.88, 5.98, and 20.03%, respectively. The mean interspecific distance is more than the mean intraspecific divergence, which indicates efficiency of DNA barcoding for identification of ascidians.

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基于COI基因序列的4种印度海鞘的鉴定

DNA条形码技术涉及线粒体DNA短片段细胞色素c氧化酶亚基1 (COI)基因的测序，是一种专门用于物种鉴定的技术，即使是在早期胚胎和幼虫阶段，这在基于形态的分类中也是相当困难的。海鞘是一种无根无脊椎脊索动物，具有多种生物化学和药理活性。在这项研究中，共有36份海鞘样本属于Didemnidae家族，对线粒体COI基因650 bp区域进行了测序。所有物种均由多个标本代表。条形码序列比对中未发现停止密码子和索引。将比对后的序列提交到条形码提交工具NCBI中，获取检索号。种内距离最小为0.00%，最大为2.23%。种内、属内和科内平均木村2参数(K2P)距离分别为0.88、5.98和20.03%。种间平均距离大于种内平均距离，表明DNA条形码对海鞘的识别效率较高。

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来源期刊

Mitochondrial Dna Part a Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.00

自引率

0.00%

发文量

期刊介绍： Mitochondrial DNA Part A publishes original high-quality manuscripts on physical, chemical, and biochemical aspects of mtDNA and proteins involved in mtDNA metabolism, and/or interactions. Manuscripts on cytosolic and extracellular mtDNA, and on dysfunction caused by alterations in mtDNA integrity as well as methodological papers detailing novel approaches for mtDNA manipulation in vitro and in vivo are welcome. Descriptive papers on DNA sequences from mitochondrial genomes, and also analytical papers in the areas of population genetics, phylogenetics and human evolution that use mitochondrial DNA as a source of evidence for studies will be considered for publication. The Journal also considers manuscripts that examine population genetic and systematic theory that specifically address the use of mitochondrial DNA sequences, as well as papers that discuss the utility of mitochondrial DNA information in medical studies and in human evolutionary biology.