Antioxidant and Esterases Profiling of diabetes-induced rat and tissues compartmentalisation.

A. Wusu
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Abstract

Enormous complications associated with diabetes contribute to the therapeutic challenge confronting most of the world, including developing countries. This study was carried out to investigate diabetes mellitus on esterases and antioxidant enzymes in different tissues compartments of rats. Animals were divided into two groups of 10 animals each. The experimental group was confirmed diabetic by a single dose of streptozotocin injection (STZ, freshly dissolved in citrate buffer, pH 4.5, 50 mg/kg) intraperitoneally. In contrast, the control group was injected with citrate buffer only. Blood glucose and weight of the animals were monitored for 7 days. Blood, liver and brain were removed, and biochemical parameters determined spectrophotometrically. Diabetes produced various degrees of alterations in antioxidant defence mechanism and esterases activities that are compartment specific. Acetylcholinesterase (AChE) activity was inhibited to various extents. While AChE was inhibited to the tune of 39% in plasma, 33% in the brain and 30% in the liver, activation of the activity was observed in the red blood cell (RBC). The same trend of significant (p < 0.001) inhibition was observed with arylesterase in the plasma, brain and liver, and activation in the RBC. Diabetes induced significant (p<0.001) inhibition in catalase, Glutathione-S-transferase, and Superoxide dismutase in the brain and liver, respectively, compared to control more than the other compartments. However, activation was also observed in the RBC of these enzymes except for catalase and nitric oxide. In conclusion, distinct compartments effects of diabetes observed in this study could suggest a new approach for effective and safer therapeutics.
糖尿病大鼠抗氧化和酯酶谱及组织区隔化。
与糖尿病相关的大量并发症使包括发展中国家在内的世界大多数国家面临治疗挑战。本研究探讨了糖尿病大鼠不同组织室中酯酶和抗氧化酶的变化。动物被分成两组,每组10只。实验组小鼠经单次腹腔注射链脲佐菌素(STZ,新鲜溶解于柠檬酸缓冲液中,pH 4.5, 50 mg/kg)证实为糖尿病。对照组只注射柠檬酸缓冲液。监测各组动物的血糖和体重,为期7 d。取血、肝、脑,分光光度法测定生化参数。糖尿病在抗氧化防御机制和酯酶活性方面产生不同程度的改变,这些改变是室特异性的。乙酰胆碱酯酶(AChE)活性受到不同程度的抑制。乙酰胆碱酯酶在血浆中被抑制39%,在脑中被抑制33%,在肝脏中被抑制30%,而在红细胞(RBC)中被激活。血浆、脑和肝脏中的芳基酯酶以及红细胞中的激活也有同样的显著抑制趋势(p < 0.001)。与对照组相比,糖尿病分别诱导大脑和肝脏过氧化氢酶、谷胱甘肽- s转移酶和超氧化物歧化酶的显著抑制(p<0.001)。然而,除过氧化氢酶和一氧化氮外,在红细胞中也观察到这些酶的活化。总之,在本研究中观察到的糖尿病不同的室室效应可能为有效和更安全的治疗方法提供新的途径。
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