{"title":"Enhanced Ethanol Production of Saccharomyces cerevisiae Induced by Cold Plasma at Atmospheric Air Pressure","authors":"X. Dong","doi":"10.5772/INTECHOPEN.78019","DOIUrl":null,"url":null,"abstract":"In this study, cold plasma at atmospheric pressure, as a novel approach of bioprocess intensification, was used to induce yeast for the improvement of ethanol production. Response surface methodology (RSM) was used to optimize the discharge-associated parameters of cold plasma for the purpose of maximizing the ethanol yield achieved by cold plasma-treated S. cerevisiae. The resulting yield of ethanol reached to 0.48 g g−1 under optimized parameters of plasma exposure time of 1 min, power voltage of 26 V, and an exposed sample volume of 9 mL, which represented an increase of 33% over control. Compared with non-exposed cells, cells exposed with plasma for 1 min presented a notable increment in cytoplasmic free Ca2+, when these exposed cells showed the significant increase in membrane potential. At the same time, ATP level decreased by about 40%, resulting in about 60% reduction in NADH. Taken together, these data suggested that the mechanism that air cold plasma raised plasma membrane potential, which led to increases in cytosolic Ca2+ concentration. Furthermore, the cofactor metabolism, such as ATP and NADH, was subjected to regulation that was mediated by Ca2+, ultimately improving yeast productivity. This may have a underlying and broad utilization in enhancing bioconversion capability of microbe in the next few years.","PeriodicalId":12484,"journal":{"name":"Fuel Ethanol Production from Sugarcane","volume":"43 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Fuel Ethanol Production from Sugarcane","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5772/INTECHOPEN.78019","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3
Abstract
In this study, cold plasma at atmospheric pressure, as a novel approach of bioprocess intensification, was used to induce yeast for the improvement of ethanol production. Response surface methodology (RSM) was used to optimize the discharge-associated parameters of cold plasma for the purpose of maximizing the ethanol yield achieved by cold plasma-treated S. cerevisiae. The resulting yield of ethanol reached to 0.48 g g−1 under optimized parameters of plasma exposure time of 1 min, power voltage of 26 V, and an exposed sample volume of 9 mL, which represented an increase of 33% over control. Compared with non-exposed cells, cells exposed with plasma for 1 min presented a notable increment in cytoplasmic free Ca2+, when these exposed cells showed the significant increase in membrane potential. At the same time, ATP level decreased by about 40%, resulting in about 60% reduction in NADH. Taken together, these data suggested that the mechanism that air cold plasma raised plasma membrane potential, which led to increases in cytosolic Ca2+ concentration. Furthermore, the cofactor metabolism, such as ATP and NADH, was subjected to regulation that was mediated by Ca2+, ultimately improving yeast productivity. This may have a underlying and broad utilization in enhancing bioconversion capability of microbe in the next few years.