Enhanced Ethanol Production of Saccharomyces cerevisiae Induced by Cold Plasma at Atmospheric Air Pressure

X. Dong
{"title":"Enhanced Ethanol Production of Saccharomyces cerevisiae Induced by Cold Plasma at Atmospheric Air Pressure","authors":"X. Dong","doi":"10.5772/INTECHOPEN.78019","DOIUrl":null,"url":null,"abstract":"In this study, cold plasma at atmospheric pressure, as a novel approach of bioprocess intensification, was used to induce yeast for the improvement of ethanol production. Response surface methodology (RSM) was used to optimize the discharge-associated parameters of cold plasma for the purpose of maximizing the ethanol yield achieved by cold plasma-treated S. cerevisiae. The resulting yield of ethanol reached to 0.48 g g−1 under optimized parameters of plasma exposure time of 1 min, power voltage of 26 V, and an exposed sample volume of 9 mL, which represented an increase of 33% over control. Compared with non-exposed cells, cells exposed with plasma for 1 min presented a notable increment in cytoplasmic free Ca2+, when these exposed cells showed the significant increase in membrane potential. At the same time, ATP level decreased by about 40%, resulting in about 60% reduction in NADH. Taken together, these data suggested that the mechanism that air cold plasma raised plasma membrane potential, which led to increases in cytosolic Ca2+ concentration. Furthermore, the cofactor metabolism, such as ATP and NADH, was subjected to regulation that was mediated by Ca2+, ultimately improving yeast productivity. This may have a underlying and broad utilization in enhancing bioconversion capability of microbe in the next few years.","PeriodicalId":12484,"journal":{"name":"Fuel Ethanol Production from Sugarcane","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2019-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Fuel Ethanol Production from Sugarcane","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5772/INTECHOPEN.78019","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3

Abstract

In this study, cold plasma at atmospheric pressure, as a novel approach of bioprocess intensification, was used to induce yeast for the improvement of ethanol production. Response surface methodology (RSM) was used to optimize the discharge-associated parameters of cold plasma for the purpose of maximizing the ethanol yield achieved by cold plasma-treated S. cerevisiae. The resulting yield of ethanol reached to 0.48 g g−1 under optimized parameters of plasma exposure time of 1 min, power voltage of 26 V, and an exposed sample volume of 9 mL, which represented an increase of 33% over control. Compared with non-exposed cells, cells exposed with plasma for 1 min presented a notable increment in cytoplasmic free Ca2+, when these exposed cells showed the significant increase in membrane potential. At the same time, ATP level decreased by about 40%, resulting in about 60% reduction in NADH. Taken together, these data suggested that the mechanism that air cold plasma raised plasma membrane potential, which led to increases in cytosolic Ca2+ concentration. Furthermore, the cofactor metabolism, such as ATP and NADH, was subjected to regulation that was mediated by Ca2+, ultimately improving yeast productivity. This may have a underlying and broad utilization in enhancing bioconversion capability of microbe in the next few years.
低温等离子体诱导酿酒酵母在常压下提高乙醇产量的研究
在本研究中,低温等离子体作为一种新的生物过程强化方法,用于诱导酵母提高乙醇产量。采用响应面法(RSM)对冷等离子体放电相关参数进行优化,以使冷等离子体处理酿酒酵母的乙醇产量最大化。在等离子体暴露时间为1 min、电源电压为26 V、暴露样品量为9 mL的优化条件下,乙醇得率达到0.48 g g−1,比对照提高了33%。与未暴露的细胞相比,暴露于血浆1min的细胞胞质游离Ca2+显著增加,膜电位显著升高。同时,ATP水平下降约40%,导致NADH降低约60%。综上所述,这些数据表明,空气冷等离子体提高质膜电位的机制,导致细胞质Ca2+浓度增加。此外,辅助因子代谢,如ATP和NADH,受到Ca2+介导的调节,最终提高酵母产量。这在提高微生物的生物转化能力方面具有潜在和广泛的应用前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信