Molecular Cloning of the cDNA of Heat Shock Protein 88 Gene from the Entomopathogenic Fungus, Paecilomyces tenuipes Jocheon-1

Abstract

Ya-qi Liu et al. Molecular Cloning of the cDNA of the Heat Shock Protein 88 Gene 72 73 present study. Until now, a great number of variable species of HSPs including small HSPs with molecular weights ranging from 15-40 kDa and many types of HSPs with molecular weights over 40 kDa have been studied; however, HSP88 is rarely investigated (Schlesinger, 1990; Sorensen et al., 2003; Davis, 2004; Burnie et al., 2006; Bendz et al., 2007; Tsan and Gao, 2009; Manjunatha et al., 2010; Shim et al., 2012; Routray et al., 2013). Although the amino acid sequence of the HSP88 has been reported from a fungal species (PlesofskyVig and Brambl, 1998), there are few reports regarding the full-length cDNA sequence. This is the first report, to our knowledge, about the HSP88 from Paecilomyces tenuipes Jocheon-1 (P. tenuipes Jocheon-1), an entomopathogenic fungus also known as the vegetable wasp, and plant worm. P. tenuipes Jocheon-1 is an ecotype of the corresponding species. Dongchoonghacho, a kind of silkworm mushroom produced from the entomopathogenic fungus of P. tenuipes, has been known as a mysterious ancient medicine in Asian countries, particularly in China, Korea, and Japan. This paper describes the molecular cloning of HSP88 cDNA from P. tenuipes Jocheon-1, the expression of cDNA, the deduced amino acid sequence of the protein, and the influence of the heat shock treatment on the corresponding mRNA expression levels. Materials and Methods