Physiological and biochemical and molecular genetic characteristics of the bacteria – the basis of the microbial preparation “Bioproductin”

Abstract

Cultures of Bacillus amyloliquefaciens BIM B-1267 D, B. mojavensis BIM B-1268 D, Priestia megaterium BIM B-1269 D and B. amyloliquefaciens BIM B-1270 D bacteria, which form the basis of the bioproduct “Bioproductin”, have mutually complementary properties – pronounced antimicrobial, nitrogen-fixing, phosphate-mobilizing and phytostimulating activities, the ability to synthesize a number of hydrolytic enzymes (proteinase, endo-1.4-β-xylanase, endo-1.4-β-glucanase, α-amylase and phytase), indolylacetic acid, siderophores. In the genomes of the studied bacterial strains, the presence of genes encoding the synthesis of bacillen, bacillibactin, bacillomycin, difficidin, phengicin, macrolactin, surfactin, and iturin was established, which confirms their significant antimicrobial potential. It is also shown that the genes determining the synthesis of indolylacetic acid – the main hormone of plant growth – are present in the bacterial chromosome.