Bioinformatics Prediction and Comparison of Artocarpus heterophyllus BRI1 Family Members

Xinrong Yang, Xudong Yu, Fanhua Wu, Zeping Cai, Jiajia Luo, P. Cao
{"title":"Bioinformatics Prediction and Comparison of Artocarpus heterophyllus BRI1 Family Members","authors":"Xinrong Yang, Xudong Yu, Fanhua Wu, Zeping Cai, Jiajia Luo, P. Cao","doi":"10.5376/mpb.2020.11.0016","DOIUrl":null,"url":null,"abstract":"Full-length trantogenic sequencing (PRJNA579273) of jackfruit seedling stems and leaves was performed. On this basis, biological information analysis was performed on Jackfruit ( Artocarpus heterophyllus ) BR INSENSITIVE 1 (BRI1) family members AhBRI1 , AhBRL1 and AhBRL2 to translate genes into proteins. Using online analysis tools, jackfruit, Arabidopsis (Arabidopsis Thaliana), Sichuan mulberry (Morus notabilis) and Poplar (Populus trichocarpa) were used to analyze and obtain different physicochemical properties data and the secondary and tertiary structure of the protein. The bioinformatics prediction and comparison between the BRI1 protein families of different species were obtained. The results showed that the amino acid residues were 1 195, 1 170 and 985, and the theoretical isoelectric points were 6.32, 5.77 and 7.08. The protein was weakly acidic, hydrophilic and stable. There are secretory pathway signal peptides or chloroplast transport peptides with transmembrane structure. The main components of the secondary structure are irregular coil and -helix, and the tertiary structure is spiral tubular structure. This study provides a basis for further functional studies on members of the BRI1 protein family of jackfruit.","PeriodicalId":32255,"journal":{"name":"Journal of Plant Molecular Breeding","volume":"13 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2020-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Plant Molecular Breeding","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5376/mpb.2020.11.0016","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Full-length trantogenic sequencing (PRJNA579273) of jackfruit seedling stems and leaves was performed. On this basis, biological information analysis was performed on Jackfruit ( Artocarpus heterophyllus ) BR INSENSITIVE 1 (BRI1) family members AhBRI1 , AhBRL1 and AhBRL2 to translate genes into proteins. Using online analysis tools, jackfruit, Arabidopsis (Arabidopsis Thaliana), Sichuan mulberry (Morus notabilis) and Poplar (Populus trichocarpa) were used to analyze and obtain different physicochemical properties data and the secondary and tertiary structure of the protein. The bioinformatics prediction and comparison between the BRI1 protein families of different species were obtained. The results showed that the amino acid residues were 1 195, 1 170 and 985, and the theoretical isoelectric points were 6.32, 5.77 and 7.08. The protein was weakly acidic, hydrophilic and stable. There are secretory pathway signal peptides or chloroplast transport peptides with transmembrane structure. The main components of the secondary structure are irregular coil and -helix, and the tertiary structure is spiral tubular structure. This study provides a basis for further functional studies on members of the BRI1 protein family of jackfruit.
异叶树BRI1家族成员的生物信息学预测与比较
对菠萝蜜幼苗茎叶进行全长基因测序(PRJNA579273)。在此基础上,对菠萝蜜(Artocarpus heterophyllus) BR不敏感1 (BRI1)家族成员AhBRI1、AhBRL1和AhBRL2进行生物信息分析,将基因转化为蛋白。利用在线分析工具,以菠萝蜜、拟南芥(Arabidopsis Thaliana)、川桑(Morus notabilis)和杨树(Populus trichocarpa)为研究对象,分析得到该蛋白的不同理化性质数据和二级、三级结构。对不同物种BRI1蛋白家族进行了生物信息学预测和比较。结果表明,氨基酸残基分别为1 195、1 170和985,理论等电点分别为6.32、5.77和7.08。该蛋白呈弱酸性、亲水性和稳定性。有分泌通路信号肽或具有跨膜结构的叶绿体转运肽。二级结构的主要构件为不规则线圈和-螺旋结构,三级结构为螺旋管状结构。该研究为进一步研究菠萝蜜BRI1蛋白家族成员的功能提供了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
审稿时长
16 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信