Antibacterial Activity of Pineapple Peel (Ananas comosus) Eco-enzyme Against Acne Bacterias (Staphylococcus aureus and Prapionibacterium acnes)

Indo. J. Chem. Res. Pub Date : 2022-01-31 DOI:10.30598//ijcr.2022.9-nin

A. Ramadani, Rizkya Karima, R. Ningrum

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引用次数: 8

Abstract

Acne (acne vulgaris) is a skin disease caused by infection or inflammation of the pilosebaceous unit. Staphylococcus aureus and Prapionibacterium acnes was the main actor in the infection. Eco-enzyme that is made from pineapple peel has been stated that have inhibitory activity against gram-positive and negative bacteria, also applied as home industry soap. This study objective as scientific support that Eco-enzyme phytochemical compounds had the antibacterial activity to the acne bacteria. Eco-enzyme was composed of pineapple peel, brown sugar, and water with the ratio of 3:1:10 for 3 months fermentation time. Eco-enzyme was screened of its phytochemical compound and antibacterial activity against S.aureus and P.acnes by dilution with various concentrations (1.5625%, 3, 125%, 6.25%, 12.5%, 25%, and 50% v/v) and various control. The minimum inhibition concentration (MIC) was then tested by diffusion method to determine the inhibition zone with 3 replication using the higher concentration (50%, 75%, 100% v/v). The result showed that eco-enzyme has a clear yellow colour, contains tannin and saponin, MIC of S.aureus and P.acnes bacteria is 50%. The dilution test of eco-enzyme gives the most effective concentration to inhibit S.aureus at eco-enzyme 100% (v/v), contrasted to P. acnes which didn’t show the best inhibition concentration.

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菠萝皮生态酶对痤疮细菌(金黄色葡萄球菌和痤疮芽孢杆菌)的抑菌活性

痤疮(寻常痤疮)是由皮脂腺单位感染或炎症引起的皮肤病。金黄色葡萄球菌和痤疮芽孢杆菌是感染的主要因素。从菠萝皮中提取的生态酶对革兰氏阳性菌和阴性菌都有抑制作用，也被应用于家庭工业肥皂中。本研究旨在为生态酶类植物化学化合物对痤疮细菌具有抑菌活性提供科学依据。以菠萝皮、红糖、水为原料，以3:1:10的比例发酵3个月。通过不同浓度(1.5625%、3、125%、6.25%、12.5%、25%和50% v/v)和不同对照稀释，筛选其植物化学成分及对金黄色葡萄球菌和痤疮假单胞菌的抑菌活性。然后用扩散法测定最小抑制浓度(MIC)，以较高的浓度(50%、75%、100% v/v)确定3次复制的抑制区。结果表明，生态酶呈明显的黄色，含有单宁和皂苷，金黄色葡萄球菌和痤疮假单胞菌的MIC为50%。生态酶稀释试验结果表明，生态酶对金黄色葡萄球菌的抑制浓度为100% (v/v)时最有效，而痤疮假单胞菌的抑制浓度不佳。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Indo. J. Chem. Res.

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