Therapy of Fermented Milk Lactobacillus Casei Strain Shirota to Level of Malondialdehyde (MDA) and Proteind Bands the Hearth the White Rats (Rattus Norvegicus) That given High Cholesterol Dietary

Abstract

Cardiovascular disease is a disease that occurs due to a disturbance in the function of the heart and blood vessels such as coronary heart disease (PJK), hypertension and stroke. One cause of PJK is a condition of hypercholesterolemia. Hypercholesterolemia is a condition which cholesterol level more than 200 mg / dL. High cholesterol dietary can cause an increase in free radicals that cause oxidative stress. Fermented milk Lactobacillus casei thought to contain biopeptida as antioxsidant. This study was to determine the effect of fermented milk Lactobacillus casei in lowering levels of MDA and repairing protein profiles of heart. Animal that used is a rat (Rattus norvegicus, males, aged 2-3 months, weightabout 100-250 gram. Rats divided into normal rats, hypercholesterolemia rats , hypercholesterolemia rats and therapy 1 mL and 2 mL . High cholesterol dietary used egg yolk, cholesterol pure and cholic acid that give in forcefeeding for 4 weeks.Therapy of Lactobacillus casei fermented milk given for 2 weeks in. Level of MDA was measured using the method of TBA and cardiac protein bands was tested using SDS-PAGE. The result of Research obtained showed Lactobacillus casei fermented milk therapy was significantly (p <0.05) lower levels of MDA and affect cardiac protein bands of rats. Doses of 2 mL is the best dose with decreased levels of MDA by 43.11% and is able to restore protein band profiles such as Lactobacillus casei normal. In conclusion tha fermented milk can be used as an alternative treatment of hypercholesterolemia