EFFECTS OF GLYCYRRHIZIN PRE-TREATMENT ON TRANSIENT ISCHEMIC BRAIN INJURY IN MICE

African Journal of Traditional, Complementary and Alternative Medicines Pub Date : 2017-10-01 DOI:10.21010/ajtcam.v14i5.15

C. Lim, Sehyun Lim, Y. Lee, B. Kong, Byoungho Lee, Chang-Hyun Kim, Bu-Yeo Kim, Suin Cho

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Abstract

Background: Ischemia-induced brain damage is the leading cause of adult disability and the fifth leading cause of death, and thus, the development of anti-apoptotic neuro-protective therapeutic agents is viewed as an attractive developmental strategy. Glycyrrhizin is the main sweet component in licorice and has a number of pharmacological activities, which include neuro-protective, anti-fungal, and anti-cariogenic activities. This study was undertaken to investigate the effects of glycyrrhizin on ischemia-induced brain damage. Materials and Methods: In infarct volumes and the levels of several apoptosis-related proteins, caspase-3, -8, 9, Bcl-xL, Bcl-2,and their activities in the brains of middle cerebral artery occlusion (MCAO) treated mice were measured using western blotting methods. Results: Single pre-treatment with glycyrrhizin (10-100 mg/kg)at 2 hours before MCAO significantly reduced infarct volumes at 24h after MCAO. In addition, glycyrrhizin effectively inhibited the activations of caspase-3 and -9 and the down-regulation of Bcl-xLprotein caused by MCAO. Conclusion: The neuro-protective effect of glycyrrhizin was found to be due to its regulation of apoptosisrelated proteins signals.The authors suggest glycyrrhizin be considered a potential candidate for the treatment of ischemia induced brain damage. Keywords: Glycyrrhizin, licorice, stroke, apoptosis

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甘草酸预处理对小鼠短暂性缺血性脑损伤的影响

背景:缺血性脑损伤是成人致残的主要原因和第五大死亡原因，因此，抗凋亡神经保护治疗剂的开发被视为一种有吸引力的发展策略。甘草酸是甘草中主要的甜味成分，具有神经保护、抗真菌和抗龋齿等药理作用。本研究旨在探讨甘草酸对缺血性脑损伤的影响。材料与方法:采用western blotting法测定大脑中动脉闭塞(MCAO)小鼠脑梗死体积及凋亡相关蛋白caspase-3、-8、9、Bcl-xL、Bcl-2的表达水平及其活性。结果:MCAO前2小时单次甘草酸预处理(10-100 mg/kg)可显著降低MCAO后24小时梗死体积。此外,甘草甜素能有效地抑制了caspase-3的激活和9的下调Bcl-xLprotein MCAO所致。结论:甘草甜素的保护神经效应被发现是由于监管apoptosisrelated蛋白质信号。作者建议甘草酸被认为是治疗缺血引起的脑损伤的潜在候选者。关键词:甘草酸，甘草，脑卒中，细胞凋亡

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来源期刊

African Journal of Traditional, Complementary and Alternative Medicines 医学-全科医学与补充医学

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The “African Journal of Traditional, Complementary and Alternative Medicines (AJTCAM)” is a peer-reviewed, multidisciplinary, international, scientific Open Access Journal that provides publication of articles on phytomedicines, ethnomedicines and veterinary ethnomedicines. The journal is published by a Non-Governmental Organization (NGO) known as “African Traditional Herbal Medicine Supporters Initiative (ATHMSI)”. The Journal welcomes submission of manuscripts that meet the general criteria of significance and scientific excellence. Papers will be published approximately two-to-three months after acceptance