Investigating the Effect of Testosterone Supplement on Heart and Muscle Damage in Rats Applied with Swimming Exercise

Ömer Kulaksiz, Sefa Lök
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引用次数: 2

Abstract

Anabolic Androgenic Steroids which are a synthetic derivative of testosterone are known to be used by some sportsmen in order to improve performance. The present study was conducted to investigate the effect of testosterone supplement on heart and muscle damage in rats applied with swimming exercise. The study was conducted on 30 day-old 27 (Wistar, male) rats that were supplied from “Selcuk University Experimental Medicine Research and Application Center”. The rats were divided into 4 groups. The rats in control groups (n:6) were fed during the study without doing any application. The rats in testosteronegroup (n:7) were supplied with a 10 mg/kg dose of testosterone 5 days a week for 3 weeks. The rats in exercise group (n:7) did freestyle swimming exercise for 30 minutes a day for 5 days a weekfor 3 weeks. The rats in testosterone + exercise group (n:7) did freestyle swimming exercise for 30 minutes and were administered with 10 mg/kg dose of testosterone supplement for 5 days a week for 3 weeks. At the end of the trial, necessary biochemical parameters were measured in serums obtained by drawing blood from tails of the rats. While levels of Serum, LDH, CK-MB, and CKwere studied by using an autoanalyzer, Troponin I level was analyzed by using ELISA kit. SPSS 15.0 packaged software was usedin statistical evaluation of the data and the results were given as mean±SE. Since the data were convenient with the normal distribution, ANOVA test was applied for betweengroups comparison and Duncan test was used to identify from which group the difference was resulted. When between-groups values of troponin I, ck-MB, and LDH, which are indicators of heart damage, and enzyme CK, which is an indicator of muscle damage,for control, exercise, testosterone, and testosterone+exercise groups were compared, a statistically significant difference was determined between control, exercise, testosterone, and testosterone+exercise groups (p<0.05). As a result of statistically analysis performed on all groups, the difference was found to be caused by testosterone and testosterone+exercise groups. Because testosterone production is naturally increased in the body during the exercise, swimming exercise and testosterone administration led to significant occurrence of troponin I, ck-MB, LDH, which are heart damage indicators in rats, and CK value, which is an indicator of muscle damage, in the blood. Keywords: Testosterone, Heart, Muscle Damage, Swimming Exercise
研究补充睾酮对游泳大鼠心脏和肌肉损伤的影响
合成代谢雄激素类固醇是一种睾酮的合成衍生物,已知一些运动员使用它来提高成绩。本研究旨在探讨睾酮补充剂对游泳运动大鼠心脏和肌肉损伤的影响。研究对象为“塞尔克大学实验医学研究与应用中心”提供的27只30日龄大鼠(雄性Wistar)。将大鼠分为4组。对照组(n:6)在研究期间饲喂,不做任何涂抹。睾酮组(n:7)给予10 mg/kg剂量的睾酮,每周5天,连续3周。运动组(n:7)进行自由泳运动,每天30分钟,每周5天,连续3周。睾酮+运动组(n:7)进行自由泳运动30分钟,并给予睾酮补充剂10 mg/kg,每周5天,连续3周。在试验结束时,通过从大鼠尾部抽血获得血清,测量必要的生化参数。采用自动分析仪检测血清、LDH、CK-MB和ck水平,ELISA试剂盒检测肌钙蛋白I水平。采用SPSS 15.0软件包对数据进行统计学评价,结果以mean±SE表示。由于数据符合正态分布,所以组间比较采用方差分析检验,并采用Duncan检验来确定差异来自哪一组。比较对照组、运动组、睾酮组、睾酮+运动组心肌损伤指标肌钙蛋白I、CK - mb、LDH和肌肉损伤指标CK的组间值,对照组、运动组、睾酮组、睾酮+运动组间差异有统计学意义(p<0.05)。通过对所有组进行统计分析,发现这种差异是由睾酮和睾酮+运动组引起的。由于运动过程中体内睾酮的产生自然增加,因此游泳运动和睾酮给药导致大鼠心脏损伤指标肌钙蛋白I、CK - mb、LDH和血液中肌肉损伤指标CK值显著升高。关键词:睾酮,心脏,肌肉损伤,游泳运动
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