Phylogeny and Molecular Study of Some Entomopathogenic Rhizobacteria Isolated from Two Regions in Algeria

Oulebsir-Mohandkaci Hakima, Benzina Farida, Khemili-Talbi Souad, M. Arezki, H. Fatma, Hadjouti Ryma
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Abstract

The use of bacteria in the control of insect pests is a form of biological control whose practice is still not widespread. It is in this context that the present work falls. It concerns the isolation, characterization and identification of local bacterial strains for the purpose of their use in the control of certain pests. Indeed, 20 bacteria were isolated from soil cultivated in the region of Boumerdes (center of Algeria) with a total of 21 bacterial strains isolated from Adrar region (Desert Algerian). After carrying out the efficacy tests against 2 insect pests; Migratory locust (Locusta migratoria) and wax moth (Galleria mellonella), 8 potentially interesting strains were identified based on their genetic traits. Molecular characterization of these strains was performed by isolation of DNA, PCR and sequencing of the 16S rRNA gene, followed by phylogenetic analysis. The rDNA16S sequences of the 8 strains named B1, B2, B3, B4, B5, B6, H1 and H2 were recorded in the EMBL/EBI database and their phylogenetic analysis revealed that they belong to the genera Bacillus, Pseudomonas, Enterobacter and Delftia with a very high percentage of similarity with Bacillus thuringiensis strains (NR_043403) (99%) for isolate B1, Bacillus weihenstephanensis (NR_024697) (99%) for isolate B2, Pseudomonas fragi (JCM5420) (99%) for isolates B3 and B4, Bacillus thuringiensis (CMBLBT-5) (99%) for isolate B4, Enterobacter ludwigii (EN-119) for B5, Bacillus thuringiensis (4916) (99%) for isolate H1 and Delftia lacustris (R-54734) (100%) for isolate H2.
阿尔及利亚两个地区分离的一些昆虫病原根细菌的系统发育和分子研究
利用细菌防治害虫是生物防治的一种形式,其做法仍未得到广泛应用。本文的工作正是在这种背景下展开的。它涉及分离、鉴定和鉴定当地菌株,以便将其用于控制某些害虫。从阿尔及利亚中部Boumerdes地区的土壤中分离出20株细菌,从阿尔及利亚沙漠地区Adrar地区分离出21株细菌。对2种害虫进行药效试验后;根据飞蝗和蜡蛾的遗传性状鉴定出8个潜在的研究品系。通过DNA分离、PCR和16S rRNA基因测序对菌株进行分子鉴定,并进行系统发育分析。系统发育分析结果表明,8株菌株分别为B1、B2、B3、B4、B5、B6、H1和H2,分别属于芽孢杆菌属、假单胞菌属、肠杆菌属和德尔菲特菌属,与分离物B1的苏云金芽孢杆菌属(NR_043403)(99%)、分离物B2的魏氏芽孢杆菌属(NR_024697)(99%)、分离物B3和B4的fragi假单胞菌属(JCM5420)(99%)具有很高的相似性。分离物B4的苏云金芽孢杆菌(CMBLBT-5) (99%), B5的路德维希肠杆菌(EN-119),分离物H1的苏云金芽孢杆菌(4916)(99%)和分离物H2的湖Delftia (R-54734)(100%)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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