Validated HPLC Method for the Determination of Gliquidone in Rat Plasma

Abstract

A HPLC method has been developed for determination of gliquidone in rat plasma. The assay involves combined extraction and precipitation with 1:1 methanol-acetonitrile, and separation of the analyte on a Shimpack ODS (C18) column with 75:25 (v/v) acetonitrile-0.1 M acetic acid as mobile phase. Detection at 229 nm was by photodiode-array detection. The assay was validated in accordance with international requirements and found to be specific, accurate and precise with a linearity range from 50 ng mL−1 to 10μg mL−1. The method was suitable for conducting pharmacokinetic studies in rats.