Gelatin and Collagen Nanofiber Scaffolds for Tissue Engineering

Abstract

One of the main complications that can present a person with second and third degree burns is the possibility of being infected by opportunistic bacteria or viruses that are present in the environment. Nowadays, the majority of the burn injuries are treated with con- ventional gauze, which involves a high probability of infection and pain for the patient being treated with this method. In order to obtain low-cost scaffolds, natural and abun - dant polymers were used such as gelatin (GEL) and collagen (COL). The GEL functions as a base scaffold, stable and flexible, and also biocompatible because it is a byproduct of the partial hydrolysis of COL, which is an indispensable component for the stability of the cell membrane and it is present in great extent in the human epithelium. nonwoven fabric, which was pre-grafted with acrylic acid or N-isopropyl acrylamide to con-struct a durable wound sandwich liner membrane with high water absorption, easy removal, and antibacterial activity in an animal skin model. The results indicated that tissue immobilized with N-isopropyl acrylamide and COL/chitosan/PP/N-isopropyl acrylamide-COL/chito-san) showed a better healing effect than COL/chitosan immobilized polypropylene tissue. The poly (propylene)/N-isopropyl acrylamide/COL/chitosan-treated wound showed an excellent remodeling effect on histological examination with respect to the construction of the vein, epi dermis, and dermis at 21 days post-cutaneous lesion. The water absorption values and water diffusion coefficient for polypropylene/N-isopropyl acrylamide/COL/chitosan were higher than those of polypropylene /acrylic acid/COL/chitosan under a weight-volume ratio of COL/ chitosan. Both polypropylene/N-isopropyl acrylamide/COL/chitosan and poly (propylene)/ acrylic acid/COL/chitosan showed antibacterial activity [20].