Molecular Analysis of Mineral and Tap Water to Detect Microbial Contaminants in Jeddah Province

Khalid Saeed A. Alzahrani, Mohammed H. Mutawakkil, Saleh M. Al-Maaqar, Nawaf M. Alharthi, M. Al-Matary, M. M. M. Ahmed
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Abstract

Since ancient times, drinking water quality has played a significant role in human diseases and infections. In Jeddah, the drinking water choices are mostly bottled water or water available in plastic containers after processing at private water treatment stations. A total of 30 drinking water samples were obtained from many sources in Jeddah, KSA: mineral water (13 samples) and tap water (17 samples). Microbiological tests were used, including a negative and positive Gram stain test for these colonies and determining the type of bacteria. To identify bacterially, Polymerase chain reaction (PCR) techniques were used in this study. Following that, the susceptibility of bacterial isolates to commonly used antibiotics is tested. The results showed that the microbial cultures were of different isolates. We confirmed that these results by microbiological examination and molecular identification found a difference between the study samples. Fifty-three bacteria were isolated from 30 samples, 34 (64.15%) tap water samples, and 19(35.85%) mineral water samples were found to be contaminated with bacteria. The highest bacterial contamination, 86.6% of all samples, was found in the tap water in 50% of samples, while 36.6% was found in mineral water. Microbiological tests and molecular diagnostic methods make it simpler and faster to shorten the detection time of drinking water contamination. The results of the current study suggest that mineral water is a better source of potable water in Jeddah than tap water. However, there may be pollution in the water at the reservoirs rather than at the water sources
吉达省矿物和自来水微生物污染物的分子分析
自古以来,饮用水质量在人类疾病和感染中起着重要作用。在吉达,饮用水的选择主要是瓶装水或在私人水处理站处理后装在塑料容器中的水。在沙特阿拉伯吉达从许多来源获得了总共30个饮用水样本:矿泉水(13个样本)和自来水(17个样本)。使用微生物试验,包括对这些菌落进行阴性和阳性革兰氏染色试验,并确定细菌的类型。本研究采用聚合酶链反应(PCR)技术进行细菌鉴定。然后,测试细菌分离物对常用抗生素的敏感性。结果表明,不同菌株的微生物培养物不同。我们通过微生物学检查和分子鉴定证实了这些结果在研究样本之间存在差异。从30份样品中分离出53种细菌,自来水样品34份(64.15%),矿泉水样品19份(35.85%)。细菌污染最高的是50%的自来水,占所有样品的86.6%,而矿泉水的细菌污染最高,占36.6%。微生物检测和分子诊断方法使饮用水污染检测更简单、更快捷。目前的研究结果表明,在吉达,矿泉水是比自来水更好的饮用水来源。然而,水库的水可能受到污染,而不是水源
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