Site–specific immobilization of Fab’ fragments of goat antihuman IgG on quartz surfaces

Abstract

This paper presents a strategy for immobilizing biomolecules onto a solid surface. We used the free thiol group directing method to immobilize Fab’ fragments to the MPTMS derived quartz substrate and results of X-ray photoelectron spectroscopic studies on the binding of MTPMS and Fab’ fragments on the quartz surfaces are reported. We also prepared a new type of immuno-labeled protein which was the rare earth element labeled antigen (human IgG) to recognize Fab’ fragment. Four characteristic peaks has been determined on the surface specifically bound human IgG labeled Tb³⁺.