Evaluation of Antioxidant potential of the Siddha Formulation Sambirani Poo Kuligai by in-vitro DPPH radical scavenging assay

Abstract

Oxidative stress has becomes a primary factor in the progression of many degenerative diseases, such as cancer, hyperlipidemia, gastric ulcer, and diabetes. Antioxidants are vital substances which possess the ability to protect body from damage caused by free radical–induced oxidative stress. Radicals and other reactive oxygen species (ROS) are formed constantly in the human body and are removed by the enzymatic and non–enzyme antioxidant defense systems. The usage of Siddha medicine has considerably increased over the past two decades and it is steadily crossing the various geographical boundaries owing to its rich antioxidant, therapeutic potential and inexpensiveness compared to conventional modern medicines and has fairly high acceptance rates because of its herbal origin and therefore its nontoxic nature.The main aim of the present investigation is to evaluate the In-vitro antioxidant potential of the novel siddha preparation Sambirani Poo Kuligai (SPK) by DPPH radical scavenging assay. The antioxidant activity of test drug sample SPK was determined using the 2,2-diphenyl 1-2 picrylhydrazyl (DPPH) free radical scavenging assay at the concentration of 1.25 μg/ml, 2.5 μg/ml,5 μg/ml,10 μg/ml and 20 μg/ml using ascorbic acid as standard. . Results of the DPPHfree radicalscavengingassay of SPKshows the highest percentage inhibition of about 82.52%at 20μg/ml when compare to standard ascorbicacid with 89.62%. In conclusion the trial drug SPK possess significant antioxidant property which contributes to the beneficial effect oxidative stress associated disorders.