Bioethanol Fermentation from Non-Treated and Pretreated Corn Stover using Aspergillus Oryzae

Chemical Engineering Research Bulletin Pub Date : 2013-12-29 DOI:10.3329/CERB.V16I1.6659

S. M. Hossain

{"title":"Bioethanol Fermentation from Non-Treated and Pretreated Corn Stover using Aspergillus Oryzae","authors":"S. M. Hossain","doi":"10.3329/CERB.V16I1.6659","DOIUrl":null,"url":null,"abstract":"A comparison was studied for non-treated and pretreated corn stover with dilute alkaline peroxide and dilute acid treatment respectively for bioethanol production by simultaneous saccharification and fermentation (SSF) process in a continuous stirred batch bioreactor using fungi Aspergillus oryzae . The optimum parameters for bioethanol fermentation were: time, 48 h; pH, 6.0; temperature, 50oC; stirring speed, 35 rpm; and corn stover loading, 35 g/L. The maximum concentration of bioethanol at optimum fermentation process parameters were 0.762 g/g, 0.799 g/g and 0.819 g/g for non-treated, dilute acid and dilute alkaline peroxide pretreated corn stover respectively. Maximum yields of bioethanol were 0.399 g/L h, 0.420 g/L.h and 0.431 g/L h of corn stover at optimum parameters for non-treated, dilute acid and dilute alkaline peroxide pretreated corn stover respectively. The sp. growth rate (m) were 5.30 s −1 , 5.54 s −1 , and 5.93 s −1 and maximum sp. growth rate (m max ) were 10.60 s −1 , 11.08 s −1 and 11.86 s −1 using Monod model for non-treated, dilute acid and dilute alkaline peroxide pretreated corn stover respectively. The Monod parameter (K s ) were 33.87 g/L, 34.21 g/L and 34.85 g/L for non-treated, dilute acid and dilute alkaline peroxide pretreated corn stover respectively. The sp. enzyme activity (ν) were 1192 min −1 , 1463 min −1 and 1559 min −1 and maximum sp. growth rate (ν max ) were 2384 min −1 , 2926 min −1 and 3118 min −1 using Michaelis-Menten enzyme kinetic model for non-treated, dilute acid and dilute alkaline peroxide pretreated corn stover respectively. The Michaelis-Menten parameter (K m ) were 34.42 g/L, 34.63 g/L and 34.85 g/L for non-treated, dilute acid and dilute alkaline peroxide pretreated corn stover respectively. The first order rate constants (k) were 0.014 h −1 , 0.016 h −1 and 0.019 h −1 for non-treated, dilute acid and dilute alkaline peroxide pretreated corn stover for continuous stirred batch bioreactor respectively. DOI: http://dx.doi.org/10.3329/cerb.v16i1.6659 Chemical Engineering Research Bulletin 16(2013) 33-44","PeriodicalId":9756,"journal":{"name":"Chemical Engineering Research Bulletin","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2013-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"6","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical Engineering Research Bulletin","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3329/CERB.V16I1.6659","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 6

Abstract

A comparison was studied for non-treated and pretreated corn stover with dilute alkaline peroxide and dilute acid treatment respectively for bioethanol production by simultaneous saccharification and fermentation (SSF) process in a continuous stirred batch bioreactor using fungi Aspergillus oryzae . The optimum parameters for bioethanol fermentation were: time, 48 h; pH, 6.0; temperature, 50oC; stirring speed, 35 rpm; and corn stover loading, 35 g/L. The maximum concentration of bioethanol at optimum fermentation process parameters were 0.762 g/g, 0.799 g/g and 0.819 g/g for non-treated, dilute acid and dilute alkaline peroxide pretreated corn stover respectively. Maximum yields of bioethanol were 0.399 g/L h, 0.420 g/L.h and 0.431 g/L h of corn stover at optimum parameters for non-treated, dilute acid and dilute alkaline peroxide pretreated corn stover respectively. The sp. growth rate (m) were 5.30 s −1 , 5.54 s −1 , and 5.93 s −1 and maximum sp. growth rate (m max ) were 10.60 s −1 , 11.08 s −1 and 11.86 s −1 using Monod model for non-treated, dilute acid and dilute alkaline peroxide pretreated corn stover respectively. The Monod parameter (K s ) were 33.87 g/L, 34.21 g/L and 34.85 g/L for non-treated, dilute acid and dilute alkaline peroxide pretreated corn stover respectively. The sp. enzyme activity (ν) were 1192 min −1 , 1463 min −1 and 1559 min −1 and maximum sp. growth rate (ν max ) were 2384 min −1 , 2926 min −1 and 3118 min −1 using Michaelis-Menten enzyme kinetic model for non-treated, dilute acid and dilute alkaline peroxide pretreated corn stover respectively. The Michaelis-Menten parameter (K m ) were 34.42 g/L, 34.63 g/L and 34.85 g/L for non-treated, dilute acid and dilute alkaline peroxide pretreated corn stover respectively. The first order rate constants (k) were 0.014 h −1 , 0.016 h −1 and 0.019 h −1 for non-treated, dilute acid and dilute alkaline peroxide pretreated corn stover for continuous stirred batch bioreactor respectively. DOI: http://dx.doi.org/10.3329/cerb.v16i1.6659 Chemical Engineering Research Bulletin 16(2013) 33-44

查看原文本刊更多论文

利用米曲霉从未处理和预处理的玉米秸秆发酵生物乙醇

以米曲霉为原料，在连续搅拌间歇式生物反应器中，对未经处理和预处理的玉米秸秆分别进行了稀碱性过氧化氢和稀酸处理，并对其糖化发酵(SSF)工艺进行了比较。生物乙醇发酵的最佳工艺参数为:发酵时间48 h;pH值6.0;温度、50摄氏度;搅拌速度，35转/分;玉米秸秆装载量为35 g/L。未处理玉米秸秆、稀酸预处理玉米秸秆和稀碱性过氧化氢预处理玉米秸秆在最佳发酵工艺条件下的最大生物乙醇浓度分别为0.762 g/g、0.799 g/g和0.819 g/g。未处理玉米秸秆、稀酸预处理玉米秸秆和稀碱性过氧化氢预处理玉米秸秆的最佳工艺条件下，生物乙醇的最大产率分别为0.399 g/L h、0.420 g/L h和0.431 g/L h。在Monod模型下，未处理、稀酸和稀碱性过氧化氢预处理玉米秸秆的sp生长速率(m)分别为5.30 s−1、5.54 s−1和5.93 s−1，最大sp生长速率(m max)分别为10.60 s−1、11.08 s−1和11.86 s−1。未处理玉米秸秆、稀酸预处理玉米秸秆和稀碱性过氧化氢预处理玉米秸秆的Monod参数(K s)分别为33.87 g/L、34.21 g/L和34.85 g/L。采用Michaelis-Menten酶动力学模型，未处理、稀酸和稀碱性过氧化氢预处理玉米秸秆的sp酶活性(ν)分别为1192、1463和1559 min−1，最大sp生长速率(ν max)分别为2384、2926和3118 min−1。未处理、稀酸和稀碱性过氧化氢预处理玉米秸秆的Michaelis-Menten参数(K m)分别为34.42 g/L、34.63 g/L和34.85 g/L。连续搅拌间歇式生物反应器中未处理、稀酸预处理和稀碱性过氧化氢预处理的玉米秸秆一级速率常数(k)分别为0.014 h−1、0.016 h−1和0.019 h−1。DOI: http://dx.doi.org/10.3329/cerb.v16i1.6659化学工程研究通报16(2013)33-44

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Chemical Engineering Research Bulletin

自引率

0.00%

发文量