Development of Cost-effective Homemade Basal Medium for Culturing Bacillus subtilis strain KPA In Vitro

Abstract

The present preliminary context was investigated to formulate a cost-effective homemade basal medium for the successful growth and subsequent culturing of Bacillus subtilis strain KPA in vitro. In view of this, PSC (Potato Soya chunk Chickpea) agar medium was prepared by adding aqueous filtered solutions of potatoes (Solanum tuberosum), soya chunks (Glycine max) and Chickpeas (Cicer arietinum) in definite proportion. This newly prepared medium favoured the growth of strain KPA and showed successful culturing after 24 h of incubation. The results observed on the agar plates were more or less similar to the growth of strain KPA on nutrient agar (NA) plates. Most importantly, the isolate grown into PSC medium showed more or less similar pattern of growth profile as in NB medium. Briefly, PSC agar medium can be used in vitro for the subsequent culturing of B. subtilis strain KPA in a cost-effective manner.