Antibacterial Effect of Matricaria chamomilla Alcoholic Extract against Drug-Resistant Isolates of Staphylococcus aureus and Pseudomonas aeruginosa

Abstract

CITATION LINKS A R T I C L E I N F O Article Type Original Research Authors Ania Ahani Azari, PhD1* Ahmad Danesh, PhD1 How to cite this article Ahani Azari A., Danesh A. Antibacterial Effect of Matricaria chamomilla Alcoholic Extract against Drug-Resistant Isolates of Staphylococcus aureus and Pseudomonas aeruginosa. Infection Epidemiology and Microbiology. 2021;7(1): 29-35 1Department of Microbiology, Gorgan Branch, Islamic Azad University, Gorgan, Iran 2Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, Iran * Correspondence Address: Department of Microbiology, Gorgan Branch, Islamic Azad University, Gorgan, Iran. ania_783@yahoo.com Article History Received: October 25 ,2020 Accepted: December 15 ,2020 Published: January 23 ,2021 Background: This study aimed to determine antibacterial activity of ethanolic extract of Matricaria chamomilla (chamomile) against methicillin-resistant Staphylococcus aureus (MRSA) and multidrug-resistant (MDR) Pseudomonas aeruginosa strains isolated from clinical specimens. Materials & Methods: The plant samples were collected, and the flowers and leaves were separated and dried completely in the shade. After grinding, extraction was performed using the maceration method. The extracts of both flowers and leaves were dried at 37°C for 24 hrs. About 500 mg of the dried plant extract was dissolved in 10 mL of 5% dimethyl sulfoxide and sterilized by filtration through a 0.45 μm membrane filter. For the antibacterial assay, agar well diffusion and broth microdilution methods were used. Findings: No inhibitory effect was observed for both extracts against MDR P. aeruginosa isolates in agar well diffusion method. In broth microdilution method, the leaves extract showed inhibitory effect, and its MIC and MBC were determined at 12.5 and 25 mg/mL concentrations, respectively. The flowers extract showed antibacterial activity against most MRSA isolates. The extract of leaves demonstrated inhibitory effect on 7 MRSA isolates. The MIC and MBC of flowers extract were determined at concentrations of 6.25 and 12.5 mg/mL for most MRSA isolates, while MIC and MBC of leaves extract were 12.5 and 25 mg/mL for a few MRSA isolates, respectively. Conclusion: In this study, the ethanolic extract of chamomile leaves showed antibacterial activity against MDR P. aeruginosa isolates; meanwhile, the flowers extract showed better activity against MRSA isolates. Copyright© 2021, TMU Press. This open-access article is published under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License which permits Share (copy and redistribute the material in any medium or format) and Adapt (remix, transform, and build upon the material) under the Attribution-NonCommercial terms. 10.29252/iem.7.1.29