Preservation of antioxidant defense system by Morin in bicalutamide-induced rat testicular toxicity

Ife Journal of Science Pub Date : 2021-05-03 DOI:10.4314/IJS.V23I1.11

E. Olayinka, A. Ore, O. Olotu, V. U. Ogbuji, O. A. Adeyemo, Olaniyi Solomon Ola

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Abstract

Bicalutamide (BCT) is a potent anti-androgen chemotherapeutic drug indicated for prostate cancer. However, BCT is known to cause oxidative stress and impairment of male reproductive function. Whereas Morin (MOR), a flavonoid has been found to be a potent antioxidant, with free radical scavenging capacity. This study investigated the protective effect of MOR on BCT-induced testicular toxicity in Wistar rats. Twenty-four male albino rats were randomized into four groups (n=6/group). Group I which served as control received distilled water. Group II, received 3 mg/kg body weight (bwt) BCT orally (per os); group III received 3 mg/kg/day BCT p.o. plus 100 mg/kg/d MOR p.o. and group IV received 100 mg/kg/d MOR p.o. All treatments lasted for 14 days, thereafter, animals were sacrificed and epididymis and testis were collected for sperm and biochemical analyses. The result revealed that BCT treatment caused a significant increase in abnormal sperm morphology. Sperm production, sperm count, motility and viability were significantly reduced when compared with control (p<0.05). Similarly, testicular superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx),glutathione S-transferase (GST) and acid phosphatase (ACP) activities, as well as ascorbic acid and GSH levels were significantly reduced in the BCT- treated animals when compared to control (p<0.05). Conversely, testicular alkaline phosphatase (ALP), gamma glutamyl transferase (GGT) and lactate dehydrogenese (LDH) activities as well as malondialdehyde (MDA) levels of BCT-treated animals increased significantly relative to control (p<0.05). However, co-treatment with Morin ameliorated BCT-induced alterations in sperm parameters, ascorbic acid, GSH and MDA levels, as well as LDH, SOD, CAT, GST, GPX, ACP, ALP and GGT activities. Data obtained from this study suggest that Morin protected against altered sperm parameters and testicular oxidative stress caused by BCT. Keywords: Bicalutamide, Anti-androgen, Testis, Oxidative stress, Morin, Antioxidant, Rat

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莫里素对比卡鲁胺致大鼠睾丸毒性的抗氧化防御系统的保护作用

比卡鲁胺(BCT)是一种有效的抗雄激素化疗药物，适用于前列腺癌。然而，已知BCT会引起氧化应激和男性生殖功能损害。而Morin (MOR)，一种类黄酮已被发现是一种有效的抗氧化剂，具有清除自由基的能力。本研究探讨了MOR对bct致Wistar大鼠睾丸毒性的保护作用。24只雄性白化大鼠随机分为4组(n=6/组)。第一组作为对照组，接受蒸馏水。II组，口服3 mg/kg体重(bwt) BCT;III组给予BCT 3 mg/kg/d + MOR 100 mg/kg/d, IV组给予MOR 100 mg/kg/d，疗程14 d，处死动物，取附睾和睾丸进行精子和生化分析。结果显示，BCT治疗导致异常精子形态显著增加。与对照组相比，精子产量、精子数量、活力和活力显著降低(p<0.05)。BCT处理动物睾丸超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)、谷胱甘肽s -转移酶(GST)和酸性磷酸酶(ACP)活性以及抗坏血酸和GSH水平均显著低于对照组(p<0.05)。相反，bct处理动物睾丸碱性磷酸酶(ALP)、谷氨酰转移酶(GGT)、乳酸脱氢酶(LDH)活性和丙二醛(MDA)水平显著高于对照组(p<0.05)。然而，与Morin共同处理可改善bct诱导的精子参数、抗坏血酸、GSH和MDA水平以及LDH、SOD、CAT、GST、GPX、ACP、ALP和GGT活性的改变。本研究获得的数据表明，桑葚胚素对BCT引起的精子参数改变和睾丸氧化应激有保护作用。关键词:比卡鲁胺，抗雄激素，睾丸，氧化应激，桑辣素，抗氧化剂，大鼠

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Ife Journal of Science

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