Features of macro- and microstructure of the liver, pancreas, stomach, small and large intestinesin the study of chronic toxicity of tablets "Trianol".

Abstract

Background. Due to the increased requirement for evaluation of safety, drugsafety and toxicological testing of new drugs take one of the most important places in pre-clinical studies. Objective: to identify features of the macro – and microstructure of the liver, pancreas, stomach, small intestine and colon in the study of chronic toxicity of tablets "Trianol". Methods. This study researched the characteristics of macro- and microstructure of the liver, pancreas, stomach, small intestine and colon in the study of chronic toxicity of tablets "Trianol". Experiments performed on rats of Vistar line of both sexes weighing 180-230g, obtained from the nursery of the Institute of Pharmacology and toxicology of NAMS of Ukraine, contained in a vivarium and receiving standard diet. When working with experimental animals was guided by the requirements of the "European Convention for the protection of vertebrate animals used for experimental and other scientific purposes" (Strasbourg, 18.03.86). Studied the toxicity of three doses of “Trianol”: minimum (therapeutic) dose for tablets amounted to 50.0 mg/kg, intermediate – 250,0 mg/kg, maximum (subtoxic) – 500,0 mg/kg. Results . It is established that chronic introduction of trianol in a therapeutic dose induces hypertrophy of the gastric mucosa and the appearance of liver hepatocytes with signs of degeneration in cytoplasm. Identified changes are reversible. Conclusion . Intermediate and subtoxic doses cause the appearance of toxic changes in the form of swelling of the mucous membrane of the stomach, degenerative changes in cells and destruction of liver cells that must be considered when prescribing the drug. Immunotoxicaction on Peyer’s patch is not revealed.