The effect of polystyrene foam in different doses on the blood parameters and relative mass of internal organs of white mice

IF 0.8 Q2 Environmental Science
M. Bilan, M. Lieshchova, V. Brygadyrenko
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引用次数: 5

Abstract

Due to their durability, versatility and economy, plastic products are widely used in all spheres of human life. Despite the inertness of polymers, recent studies show the ability of microplastic to overcome natural tissue barriers, accumulate in the animal’s body, affect metabolism and change the intestinal microbiota, negatively affecting it. In a 42-day experiment, changes in the internal organs’ relative mass, blood biochemical and morphological parameters of white mice were established under the influence of different doses of polystyrene foam in their diet. Four groups of white mice consumed crushed polystyrene foam particles (10%, 1% and 0.1% by weight of the feed, control group without the addition of polystyrene foam). At the end of the experiment, the morphofunctional state of the internal organs was determined by the organ mass index and blood biochemical parameters. Adding crushed polystyrene foam to the feed in an amount of 1% causes a significant decrease in the mass index of the heart and stomach, 10% – only the heart, and 0.1% – does not affect this indicator. Polystyrene foam had a significant effect on blood biochemical parameters, regardless of the dose, causing an increase in the activity of aspartate aminotransferase against the background of a decrease in the activity of alkaline phosphatase. The content of total bilirubin, urea, urea nitrogen and cholesterol decreased, and the concentration of creatinine and total protein increased (due to the albumin fraction). The use of crushed polystyrene foam in mice did not cause significant changes in the blood morphological composition, except for a dose-dependent increase in the number of monocytes. In the future, it is planned to determine histological, histochemical and immunohistochemical changes in the organs of laboratory animals under the influence of plastic in a laboratory experiment.
不同剂量聚苯乙烯泡沫对小白鼠血液参数及内脏相对质量的影响
由于其耐用性、通用性和经济性,塑料制品被广泛应用于人类生活的各个领域。尽管聚合物是惰性的,但最近的研究表明,微塑料能够克服天然组织屏障,在动物体内积累,影响代谢并改变肠道微生物群,对其产生负面影响。在42天的实验中,研究了不同剂量的聚苯乙烯泡沫对小白鼠内脏相对质量、血液生化和形态学参数的影响。四组小白鼠食用粉碎的聚苯乙烯泡沫颗粒(饲料重量的10%、1%和0.1%,对照组不添加聚苯乙烯泡沫)。实验结束时,通过脏器质量指数和血液生化指标测定脏器形态功能状态。在饲料中添加1%的聚苯乙烯泡沫碎料会使心脏和胃的质量指数显著下降,10%只对心脏有影响,0.1%对该指标没有影响。无论剂量如何,聚苯乙烯泡沫对血液生化参数都有显著影响,导致天冬氨酸转氨酶活性升高,而碱性磷酸酶活性降低。总胆红素、尿素、尿素氮和胆固醇含量降低,肌酐和总蛋白浓度升高(由于白蛋白部分)。在小鼠中使用破碎的聚苯乙烯泡沫并没有引起血液形态组成的显著变化,除了单核细胞数量的剂量依赖性增加。未来,计划在实验室实验中确定塑料影响下实验动物器官的组织学、组织化学和免疫组织化学变化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
2.40
自引率
0.00%
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0
审稿时长
12 weeks
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