The Longevity of Sexed Sperm after Sexing with Several Combination of Bovine Serum Albumin (BSA) Concentration

Advances in Biological Sciences Research Pub Date : 2022-01-01 DOI:10.2991/absr.k.220309.029

N. Solihati, S. Rasad, N. Hilmia

{"title":"The Longevity of Sexed Sperm after Sexing with Several Combination of Bovine Serum Albumin (BSA) Concentration","authors":"N. Solihati, S. Rasad, N. Hilmia","doi":"10.2991/absr.k.220309.029","DOIUrl":null,"url":null,"abstract":"The aim of the research was to evaluate the longevity of sexed sperm of local ram after sexing with different combination of Bovine Serum Albumin (BSA) concentration in chilled and post thawed sexed sperm. The object of the research was 10 ejaculated semen from 3 years old local ram. The method was a Completely Randomize Design (CRD) with four treatments combination of BSA concentration on upper layer and bottom layer (T1: 3% and 6%, T2: 4% and 8%, T3: 5% and 10%, dan T4: 6% and 12%), each treatment was repeated 10 times. Result showed that there is significant difference (P<0.05) in sperm longevity both in chilled and post thawed sexed sperm. Sperm longevity in chilled sexed sperm on of upper layer in T1, T2, T3 and T4 were 229.60±16.13, 231.90±10.27, 265.40±15.01, and 196.60±19.21 hours, respectively, as well as on bottom layer in T1, T2, T3 and T4 were 207.70±31.18, 207.90±31.47, 239.10±30.82 and 181.60±31.81 hours, respectively. Sperm longevity in post thawed sexed sperm on upper layer in T1, T2, T3 and T4 were 142.80±2.68, 142.20±1.64, 147.00±3.00, and 142.20±1.64 hours, respectively, and then on bottom layer were 121.80±2.68, 120.60±3.29, 125.40±3.29 and 118.80±1.64 hours, respectively. In conclusion, the combination of BSA concentration of 5% and 10% at upper and bottom layer resulting longest sperm longevity both in chilled and post thawed sexed sperm.","PeriodicalId":7202,"journal":{"name":"Advances in Biological Sciences Research","volume":"728 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advances in Biological Sciences Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2991/absr.k.220309.029","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

The aim of the research was to evaluate the longevity of sexed sperm of local ram after sexing with different combination of Bovine Serum Albumin (BSA) concentration in chilled and post thawed sexed sperm. The object of the research was 10 ejaculated semen from 3 years old local ram. The method was a Completely Randomize Design (CRD) with four treatments combination of BSA concentration on upper layer and bottom layer (T1: 3% and 6%, T2: 4% and 8%, T3: 5% and 10%, dan T4: 6% and 12%), each treatment was repeated 10 times. Result showed that there is significant difference (P<0.05) in sperm longevity both in chilled and post thawed sexed sperm. Sperm longevity in chilled sexed sperm on of upper layer in T1, T2, T3 and T4 were 229.60±16.13, 231.90±10.27, 265.40±15.01, and 196.60±19.21 hours, respectively, as well as on bottom layer in T1, T2, T3 and T4 were 207.70±31.18, 207.90±31.47, 239.10±30.82 and 181.60±31.81 hours, respectively. Sperm longevity in post thawed sexed sperm on upper layer in T1, T2, T3 and T4 were 142.80±2.68, 142.20±1.64, 147.00±3.00, and 142.20±1.64 hours, respectively, and then on bottom layer were 121.80±2.68, 120.60±3.29, 125.40±3.29 and 118.80±1.64 hours, respectively. In conclusion, the combination of BSA concentration of 5% and 10% at upper and bottom layer resulting longest sperm longevity both in chilled and post thawed sexed sperm.

查看原文本刊更多论文

不同牛血清白蛋白(BSA)浓度组合对有性生殖后精子寿命的影响

本研究旨在通过不同的牛血清白蛋白(BSA)浓度组合对本地公羊有性精子进行性别鉴定后的寿命进行评价。研究对象为当地3岁公羊的10枚射精精液。方法采用完全随机设计(CRD)， 4个处理(T1: 3%和6%，T2: 4%和8%，T3: 5%和10%，T4: 6%和12%)，每个处理重复10次。结果表明，冷冻和解冻后的有性精子寿命差异有统计学意义(P<0.05)。T1、T2、T3、T4低温性精子上层精子寿命分别为229.60±16.13、231.90±10.27、265.40±15.01、196.60±19.21小时，T1、T2、T3、T4低温性精子下层精子寿命分别为207.70±31.18、207.90±31.47、239.10±30.82、181.60±31.81小时。T1、T2、T3、T4期上层解冻后性精子寿命分别为142.80±2.68、142.20±1.64、147.00±3.00、142.20±1.64小时，下层解冻后性精子寿命分别为121.80±2.68、120.60±3.29、125.40±3.29、118.80±1.64小时。综上所述，上层和底层BSA浓度分别为5%和10%时，冷冻和解冻后的有性精子寿命最长。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Advances in Biological Sciences Research

自引率

0.00%

发文量