Comparative proteome analysis in hot pepper (Capsicum annuum L.) after space flight

IF 1.3 4区生物学 Q3 PLANT SCIENCES

Phyton-international Journal of Experimental Botany Pub Date : 2017-12-21 DOI:10.32604/phyton.2017.86.236

L. Xie, X. Wang, M. Peng, Y.G. Zhou, Lx Chen, Lx Liu, Yl Gao, Yh Guo

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引用次数: 5

Abstract

Hot pepper (Capsicum annuum L.) is an important cropall over the world. To explore and identify differentially expressedproteins of hot pepper after space flight, three space-induced mutants(Y1, Y2 and Y3), which obtained new traits after space flightcompared with their control lines (W1 and W2), were analyzed usingcomparative proteome analysis. In this study, leaf morphologicalcharacteristics of five kinds of hot pepper variations were evaluatedby scanning electron microscopy (SEM) and transmission electronmicroscopy (TEM). Results showed that obvious changes of cellularstructure were seen in space induced mutants. Thirty nine out ofthe 255 differentially expressed proteins were successfully sequenced.Among these 39 proteins, 31 were homologous with known proteins,and the others were set as a hypothetical protein. All identifiedproteins were further classified into six groups including proteinmetabolism, energy metabolism and photosynthesis. In conclusion,these findings will benefit for the research of mutagenic mechanismsat the level of proteomics, and provide an effective way for new hotpepper breeding.

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航天后辣椒(Capsicum annuum L.)蛋白质组比较分析

辣椒(Capsicum annuum L.)是世界上重要的农作物。为了探索和鉴定航天后辣椒的差异表达蛋白，采用比较蛋白质组学分析方法对航天后获得新性状的3个航天诱导突变体(Y1、Y2和Y3)进行了分析，并与对照系(W1和W2)进行了比较。采用扫描电子显微镜(SEM)和透射电子显微镜(TEM)对5种辣椒品种的叶片形态特征进行了研究。结果表明，空间诱导突变体的细胞结构发生了明显的变化。255个差异表达蛋白中有39个成功测序。在这39个蛋白中，31个与已知蛋白同源，其余被设置为假设蛋白。所有鉴定的蛋白质进一步分为6类，包括蛋白质代谢、能量代谢和光合作用。本研究结果将有助于在蛋白质组学水平上研究辣椒诱变机理，并为辣椒新品种选育提供有效途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Phyton-international Journal of Experimental Botany 生物-植物科学

CiteScore

1.90

自引率

11.80%

发文量

审稿时长

12 months

期刊介绍： Phyton-International Journal of Experimental Botany is an international journal that publishes on the broadest aspects of plant biology and ecology. The journal welcomes the original and exciting submissions that provide new and fundamental insights into the origins, development, and function of plants from the molecular to the whole organism and its interactions within the biotic and abiotic environment. Phyton-International Journal of Experimental Botany publishes outstanding research in the plant and ecology sciences, especially in the areas of plant physiology and biochemistry, plant metabolism, plant ecology and evolution, as well as those making use of synthetic, modeling, bioinformatics, and -omics tools. Manuscripts submitted to this journal must not be under simultaneous consideration or have been published elsewhere, either in part or in whole.