Analysis of Plasma Fluorescence Spectra Mechanism

Abstract

the UV-induced plasma fluorescence spectra are obtained by FLS920 Spectrometer. There is a stronger and wider peak from 300nm to 410nm in the plasma fluorescence spectrum. Using soft analysis, it is found that the peak is actually consisted of two Gaussion curves, or two fluorophores, which are albumin and immunoglobulin (Ig). And their rate is about 58% and 37% completed. We are thought that the Albumin fluorescence is most, but the fluorescence information of Ig is more important for medical diagnoses. Abnormity of immune system may change Ig construction, and its spectral characteristics is altered. Therefore, based on UV-induced plasma spectra, Ig spectra can be indirectly studied by mathematical analysis. KeywordsUV-fluorescence, plasma, albumin, immunoglobulin (Ig)