Effect of Carica papaya Leaf Juice on Blood Cell Count of Busulfan-Induced Chronic Bone Marrow Aplasia in Mice

Proceedings of Shaikh Zayed Medical Complex Lahore Pub Date : 2021-11-08 DOI:10.47489/pszmc-815354-30-35

Sofia Yasmeen Abbasi

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Abstract

Introduction: Aplastic anemia is a syndrome of bone marrow failure comprising of peripheral pancytopenia and marrow hypoplasia. Recently, research has shown papaya leaf juice to increase blood cells counts. Aims & Objectives: This research was planned to investigate the effect of papaya leaf juice on blood cell counts in busulfan induced myelosuppressed mice. Place and duration of study: Post Graduate Medical Institute, Lahore from September 2015 to December 2015. Material &Methods: This animal experimental study comprised of 36 adult healthy Swiss albino mice weighing 30-35 g, divided randomly into three equal groups. Busulfan was given orally (14mg/kg) to induce myelosuppression to busulfan group and Carica papaya+ busulfan group on 8 occasions over a period of 23 days. Carica papaya leaf juice (10ml/kg) was given to the Carica papaya+ busulfan group daily for 23 days to observe its role in preventing myelosuppression. Normal group was given distilled water in an equivalent amount. One day after the final dose of busulfan and Carica papaya, the animals were sampled for blood cell counts. Data was analyzed using SPSS version 20. A p-value < 0.05 and below was considered as significant. Results: The Carica papaya leaf juice not only prevented significant fall in hemoglobin (10.86 ± 1.455 g/dl), RBC (9.54 ± 0.954× 106/mm3) and platelet count (958.92 ± 176.22 x103/mm3) vs (9.70 ± 0.904,11.24 ± 0.952 g/dl), RBC count (6.5025 ± 1.43,10.43 ± 0.234× 106/mm3) and platelet count (627.83 ± 151.63, 3881.08 ± 83.13 x103/mm3) in busulfan group but also kept them close to normal range. Total leukocyte count in C.papaya treated group improved significantly to 8116.67± 1540.85/mm3 vs (4516.67±789.51, 6666.67±798.10/mm3) busulfan treated and normal groups respectively. Conclusion: The Carica papaya leaf juice possesses significant myeloprotective activity. It may be a good candidate for prevention of chemotherapy induced pancytopenia

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番木瓜叶汁对布苏凡诱导的慢性骨髓发育不全小鼠血细胞计数的影响

再生障碍性贫血是一种骨髓衰竭综合征，包括外周血全血细胞减少和骨髓发育不全。最近，研究表明木瓜叶汁可以增加血细胞计数。目的:本研究旨在探讨番木瓜叶汁对布苏凡诱导骨髓抑制小鼠血细胞计数的影响。学习地点和时间:2015年9月至2015年12月，拉合尔研究生医学研究所。材料与方法:选用体重30 ~ 35 g的健康成年瑞士白化小鼠36只，随机分为3组。布苏凡组和番木瓜+布苏凡组分别口服布苏凡(14mg/kg) 8次，诱导骨髓抑制，疗程23 d。每天给予番木瓜叶汁10ml/kg，连续23 d观察番木瓜叶汁对骨髓抑制的预防作用。正常组给予等量蒸馏水。在服用最后剂量的布苏凡和番木瓜一天后，对这些动物进行血细胞计数取样。数据分析采用SPSS version 20。p值< 0.05及以下为显著性。结果:番木瓜叶汁不仅使血红蛋白(10.86±1.455 g/dl)、红细胞(9.54±0.954× 106/mm3)、血小板计数(958.92±176.22 x103/mm3)、红细胞计数(6.5025±1.43、10.43±0.234× 106/mm3)和血小板计数(627.83±151.63、3881.08±83.13 x103/mm3)明显下降，而且使其接近正常范围。木瓜处理组白细胞总数为8116.67±1540.85/mm3，显著高于常规处理组(4516.67±789.51,6666.67±798.10/mm3)。结论:番木瓜叶汁具有明显的骨髓保护作用。它可能是预防化疗引起的全血细胞减少症的一个很好的候选药物

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Proceedings of Shaikh Zayed Medical Complex Lahore

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