Supramolecular methods: the 8-hydroxypyrene-1,3,6-trisulfonic acid (HPTS) transport assay

IF 2.1 4区化学 Q3 CHEMISTRY, MULTIDISCIPLINARY

Supramolecular Chemistry Pub Date : 2021-07-03 DOI:10.1080/10610278.2021.1999956

Alexander M Gilchrist, Patrick Wang, Israel Carreira-Barral, Daniel Alonso-Carrillo, Xin Wu, R. Quesada, Philip A. Gale

引用次数: 10

Abstract

ABSTRACT The liposomal membrane transport assay, which uses 8-hydroxypyrene-1,3,6-trisulfonic acid to monitor the internal pH of the vesicles (the HPTS assay), is a widely used technique for analysing the activity of anionophore-facilitated transport across a phospholipid membrane. This paper describes the stepwise technique to conduct this transport assay, detailing both the perks and pitfalls of using this method to determine the activity of an anionophore and the transport mechanism. GRAPHICAL ABSTRACT

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超分子方法:8-羟基芘-1,3,6-三磺酸(HPTS)转运试验

脂质体膜转运试验，使用8-羟基芘-1,3,6-三磺酸来监测囊泡的内部pH值(HPTS试验)，是一种广泛使用的技术，用于分析阴离子载体促进转运磷脂膜的活性。本文描述了进行这种运输试验的逐步技术，详细说明了使用这种方法来确定阴离子载体的活性和运输机制的优点和缺陷。图形抽象

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Supramolecular Chemistry 化学-化学综合

CiteScore

3.60

自引率

3.00%

发文量

审稿时长

2.7 months

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