Ultra-thin, evaporation-resistent PDMS devices for absolute quantification of DNA using digital PCR

Abstract

We present an array-based nanoliter digital PCR (dPCR) platform. The ultra-thin microfluidic device can accommodate high temperatures for extended periods, while minimizing evaporation. The device utilizes a very simple fabrication scheme, and can achieve a limit of detection of 100 attomolar of synthetic target. dPCR presents a feasible way to quantify rare targets, especially in high background. We demonstrate proof-of-concept use of this device to identify methylation of synthetic DNA in high background, a potential biomarker for many diseases. We believe that this simple and cost-effective device will encourage wider adoption of dPCR.