Identification and analysis of microRNAs in Botryococcus braunii using high-throughput sequencing

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2017-02-21 DOI:10.3354/AB00672

Xiangyuan Deng, Xiao-li Hu, Da Li, Ling Wang, Jie Cheng, Kun Gao

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引用次数: 5

Abstract

MicroRNAs (miRNAs) play important regulatory roles in the growth and development of organisms. The colonial green microalga Botryococcus braunii is an oil-rich algae and little is known about its miRNAs and their target genes. Here we constructed and sequenced a small RNA library for B. braunii using the HiSeq 2000 deep sequencing method. In total, we identified 42 known miRNA families and 14 novel miRNAs from B. braunii via sequence alignment and secondary structure prediction. Quantitative real-time PCR analysis indicated that the majority of miRNAs were effective and credible. Gene ontology analysis showed that most of the targets of miRNAs were classified as being involved in metabolic and cellular processes, gene expression regulation and stress/defense functions. Our findings provide the first large-scale identification and characterization of B. braunii miRNAs and their potential target genes. This study could lead to further identification of B. braunii miRNAs and enhance our understanding of their regulatory mechanisms in diverse biological and metabolic processes.

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利用高通量测序技术鉴定和分析脑杆菌球菌microrna

MicroRNAs (miRNAs)在生物体的生长发育中起着重要的调控作用。群居的绿色微藻布朗杆菌属(Botryococcus braunii)是一种富油藻类，对其mirna及其靶基因知之甚少。本研究利用HiSeq 2000深度测序方法构建了一个小RNA文库，并对其进行了测序。通过序列比对和二级结构预测，共鉴定出42个已知的miRNA家族和14个新的miRNA家族。实时荧光定量PCR分析表明，大多数mirna是有效的、可信的。基因本体论分析表明，mirna的靶点大多被归类为参与代谢和细胞过程、基因表达调控和应激/防御功能。我们的研究结果首次大规模鉴定和表征了布劳尼贝氏杆菌mirna及其潜在靶基因。该研究有助于进一步鉴定布劳尼贝氏杆菌的mirna，并加深我们对其在多种生物和代谢过程中的调控机制的理解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.