Antioxidant and inflammatory cytokines regulatory actions of fresh snail and seawater gastropods extracts

IF 0.2 Q4 PHARMACOLOGY & PHARMACY
Deepak P. Pawar, P. Shamkuwar
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引用次数: 1

Abstract

Context: Mollusca also termed mollusks. These are unsegmented, bilateral animals. Freshwater and seawater gastropods are considered rich source of bioactive molecules that possesses various therapeutic potential. Aim: The present work aimed to investigate the antioxidant, wound healing, and anti-inflammatory effects along with the regulation of inflammatory protein at the cellular level by apple snail (freshwater gastropod) and tibia shell (seawater gastropod) extract. Materials and Methods: The mass of freshwater and seawater gastropods was macerated in water. Results: The aqueous extract of apple snail exhibited the highest 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and 2,2'-azinobis-(3-ethylbenzothiazoline)-6-sulphonic acid (ABTS) activity at 85.62% and 73.35% at 10 mg/ml, whereas the aqueous extract of seawater gastropod exhibited the highest DPPH radical scavenging activity and ABTS activity 83.53% and 76.14% at 1 mg/ml. Marine water snail aqueous extract and freshwater snail aqueous extract, each of 250 mg/kg showed 83% and 67.14% inhibition of paw edema, respectively. The cell viability study was performed by MTT assay and both the extracts showed cell viability above 97%. Both samples (freshwater snail extract and seawater gastropod extract) have good anti-inflammatory effects as they lower the concentration of tumor necrosis factor-alpha (pro-inflammatory cytokines) and increase interleukin-10 (anti-inflammatory cytokines). Conclusion: The present work concluded that the extracts of seawater gastropods were found to be more effective as an antioxidant and anti-inflammatory agent.
新鲜蜗牛和海水腹足动物提取物的抗氧化和炎症细胞因子调节作用
背景:软体动物也被称为软体动物。它们是无节的,两侧的动物。淡水和海水腹足动物被认为是生物活性分子的丰富来源,具有多种治疗潜力。目的:研究苹果蜗牛(淡水腹足动物)和胫骨壳(海水腹足动物)提取物在细胞水平上的抗氧化、创面愈合和抗炎作用以及对炎症蛋白的调节作用。材料与方法:将淡水腹足动物和海水腹足动物浸泡在水中。结果:苹果蜗牛水提液在10 mg/ml浓度下对DPPH自由基和ABTS自由基的清除率最高,分别为85.62%和73.35%;海水腹足动物水提液在1 mg/ml浓度下对DPPH自由基和ABTS自由基的清除率最高,分别为83.53%和76.14%。海水螺水提物和淡水螺水提物浓度为250 mg/kg时,对足跖水肿的抑制作用分别为83%和67.14%。MTT法测定细胞活力,两种提取物的细胞活力均在97%以上。两种样品(淡水蜗牛提取物和海水腹足动物提取物)均具有较好的抗炎作用,可降低肿瘤坏死因子- α(促炎细胞因子)浓度,增加白细胞介素-10(抗炎细胞因子)浓度。结论:海水腹足动物提取物具有较好的抗氧化和抗炎作用。
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