Interleukin-23R (rs11209026) polymorphism confer the susceptibility in Psoriasis patients

Abstract

Background: The recent advances in the understanding of psoriasis pathogenesis have clarified the pivotal role of interleukin (IL)-23. IL-23 is a heterodimeric cytokine, consisting of two subunits, the unique p19 and p40, which are shared with IL-12.The basic role of IL-23 in psoriasis is the activation and maintenance of the T-helper17 pathway.IL23R is a key player in the proliferation and survival of Th17 cells.The IL23 axis is the protagonist of the immunopathogenesis of psoriasis.therefore present study suggests to evaluated and investigated the single nucleotide polymorphisms of IL-23R (rs11209026) gene in seventy subjects.This study was find out the significant importance of IL-23R rs11209026 in disease susceptibility. Material and Methods: A blood sample was collected from two groups. The first group was patients with Psoriasis; the second group was healthy volunteers. Blood of this sample was collected in ethylinditetracitic acid (EDTA) tube and stored at 4°C for DNA extraction, polymerase-chain reaction -Restraction Fragment Polymorphism in length (PCR-RFLP) method which used to study SNP of IL-23R (rs11209026) gene. Results: The current study showed the range age of patients was (14–60), the results also revealed that 45.7% of the patients were males, and 54.3% of the patients were females.Regarding the IL-23R gene rs11209026 single nucleotide polymorphism in Iraqi psoriasis patients frequency distribution of genotype GG was significantly more frequent in patients group than in control, Patients were distributed as 7 (10.0 %) with allele A and 63 (90.0 %) with allele G; whereas, control group included 21 (30.0 %) individuals with allele A and 49 (70.0 %) subjects with allele G.