Determination of glyphosate herbicide and (aminomethyl)phosphonic acid (AMPA) in water by liquid chromatography and fluorescence detection. Part II: Direct determination using pre-column derivatization with NBD-Cl.

Abstract

An analytical method was developed for the determination of glyphosate and AMPA in water. This method involves derivatization in situ with NBD-Cl reagent at 60 °C. The quantification is performed using liquid chromatography with fluores- cence detection after a separation on an amino column. The limit of quantification was estimated at 1 µg/L level for glyphosate and at 0.1 µg/L level for AMPA.