Screening for Bioflocculant-Producing Bacteria from the Marine Environment of Sodwana Bay, South Africa

A. Ugbenyen, J. Simonis, A. Basson
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引用次数: 10

Abstract

Abstract Flocculants are chemicals that mediate flocculation process, by aggregating colloids from suspension to form floc. Chemical flocculants are hazardous to the environment, which inform the search for safer and eco-friendly alternatives from microorganisms. Bacterial strains were isolated from water and sediment samples collected from Sodwana Bay, South Africa, and physiological properties of the bacterial strains were observed. Flocculation test using kaolin clay suspension was done on all isolates and the ones that showed flocculating activity were identified molecularly using 16 rRNA gene sequence analysis. Forty marine bacteria isolates were gotten from sediments and water samples collected from Sodwana Bay. Most of the isolates exhibited a range of colony pigmentation (pink, creamy, yellow, and white). After purification of individual isolates, they were screened for their potential to produce bioflocculant. The result revealed that isolates marked SOD3, SOD10, SOD12, SOD26, SOD27, SOD28, SOD32, SOD33 and SOD34 produced bioflocculants as shown by the flocculating activities from their crude extract. All these isolates showed good flocculation of kaolin clay suspension above 60% (flocculating activity) except SOD12. These bioflocculant producing isolates were identified as Pseudoalteromonas sp, Alcaligenes faecalis, Bacillus subtilis, Bacillus cereus, Bacillus stratosphericus. The results showed Sodwana Bay, South Africa as a reservoir of bacteria with potential to produce flocculants. However, further studies on the optimisation of culture conditions for bioflocculant production, extraction, characterisation and application of isolates is on the way to underscore the biotechnological importance of these microbes as producers of substitutes to harmful chemical flocculants commonly used in water and wastewater treatment.
南非Sodwana湾海洋环境中产絮凝剂细菌的筛选
摘要絮凝剂是通过将悬浮液中的胶体聚集成絮凝体,介导絮凝过程的化学物质。化学絮凝剂对环境有害,因此需要寻找更安全、更环保的微生物替代品。从南非Sodwana湾的水和沉积物样品中分离出菌株,并观察菌株的生理特性。采用高岭土悬浮液对各菌株进行絮凝试验,并利用16rrna基因序列分析对具有絮凝活性的菌株进行分子鉴定。从索瓦纳湾的沉积物和水样中分离出40株海洋细菌。大多数分离株表现出一系列的菌落色素沉着(粉红色、奶油色、黄色和白色)。分离株纯化后,筛选其生产生物絮凝剂的潜力。结果表明,标记为SOD3、SOD10、SOD12、SOD26、SOD27、SOD28、SOD32、SOD33和SOD34的分离菌株对其粗提物具有絮凝活性,可产生生物絮凝剂。除SOD12外,其余菌株对高岭土悬浮液的絮凝活性均在60%以上(絮凝活性)。这些产生生物絮凝剂的菌株被鉴定为假互变单胞菌、粪碱性芽孢杆菌、枯草芽孢杆菌、蜡样芽孢杆菌和平流层芽孢杆菌。结果表明,南非的Sodwana湾是一个具有生产絮凝剂潜力的细菌储存库。然而,对生物絮凝剂生产、提取、分离物表征和应用的培养条件优化的进一步研究正在进行中,以强调这些微生物作为水和废水处理中常用的有害化学絮凝剂替代品的生物技术重要性。
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