The role of dietary lignocellulose inclusion with or without probiotic supplementation on some immune and blood biochemical parametres in broiler chickens

Abstract

Two hundred and forty, one-day old Avian broiler chicks were used to investigate The immune role of dietary Lignocellulose (LC) inclusion with or without probiotic supplementation and their effect on some blood biochemical parameters in broiler chickens. Experimental treatments consisted of 3×2 factorial arrangements with 3 levels of LC without or with probiotic to formulate six different experimental groups. Groups 1, 3, and 5 were fed on the basal diet with dietary LC inclusion at 0, 0.5%, and 1.0% respectively, while groups 2, 4 and 6 were fed on the previously mentioned design with probiotic at 100gm/ton. The obtained results revealed that dietary inclusion of either LC or probiotic increased (P≥0.05) WBCs count, LC combination with probiotic majored the WBCS compared with LC free groups. LC inclusion without probiotic reduced (P≥0.05) RBCs count, Hb and PCV. Meanwhile probiotic supplementation in combination with LC non-significantly improved these 3 parameters compared with probiotic free groups. Dietary inclusion of LC and / or probiotic minify lymphocyte (p<0.05) but increased (P≥0.05) heterophil %, phagocytic and phagocytic index and level of the antibody titer against Newcastle disease. LC and / or probiotic addition led to better serum lipid profile (decreased LDL, VLDL and CHO/HDL and increased HDL) and resulted in elevation of serum glucose. dietary inclusion of either LC or probiotic had no significant effect on kidney and liver function related enzymes. The probiotic supplemented diet with 0.5% LC inclusion recorded the lowest serum creatinine level. We concluded that either LC or probiotic enhanced immune response parameters and lipid profile (increased HDL and lowered cholestrol) and their combination magnified this enhancement. LC (0.5% - 1.0%) with probiotic supplemented diet revealed the best blood picture and lipid profile, the highest heterophil %.