In-vitro Antacid Properties of Cameroonian Clay (MY41g) and its Potential Use in Anti-ulcer Triple Therapy Regimen Formulated with Eremomastax speciosa Extract

J. Emakoua, Mesmine Kuissu Teukam Mimosette, A. P. Amang, Mbida Désirée Essama, Otto Gustave Lebeau Ndji, C. Mezui, Enow-Orock George Enonchong, P. V. Tan
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Abstract

Aims: The antisecretory, antibacterial on Helicobacter, and antacid properties of Eremomastax speciosa ( E. speciosa ) and MY41g clay respectively, led us to evaluate the potential use of this clay in the anti-ulcer tri-therapy formulated with Eremomastax speciosa. and Materials and Methods: In vitro antacid were evaluated by studying: Fordtran's method, and the influence of temperature on the pH values. I n vivo activity was studied on chronic gastric ulcers induced by injection of 0.05 ml of acetic acid (30%) into the stomach wall. Rats were treated daily for 10 days after ulcer induction with a combination of E. speciosa and MY41g (ESMY) ; (ESMY 100+250 and 200+250 mg/kg). The model of "unhealed" gastric ulcers was also used: from day 5 to day 18 of experimentation, rats were given ESMY orally concomitantly with indomethacin (1 mg/kg/day) subcutaneously. Ulcer index, percentage of healing, mucus secretion, gastric acidity, histological, hematological, and oxidative stress parameters were evaluated. Results: ESMY showed good neutralizing capacity in vitro in Fordtran’s method. Treatment with ESMY accelerated the spontaneous healing of chronic gastric ulcers (93.82-96.14%). However, administration of indomethacin did not induce significant variations in the percentage of healing (90.73-94.60%). For both ulcer models performed, ulcer healing was accompanied by a significant ( P = 0.001) increase in mucus mass at 200/250 mg/kg. ESMY increased antioxidant activity, decreased gastric acidity, lipid peroxidation, and maintained hematological balance. Conclusion: In addition to its buffering properties, the healing mechanism of ESMY includes reduced gastric acidity, enhanced mucus production, re-epithelialization of gastric mucosa, improvement of hematological and antioxidant status. ESMY can be used in traditional medicine, as a therapeutic regimen against gastric ulcers.
喀麦隆粘土(MY41g)的体外抗酸性能及其在用白藜芦醇提取物配制的抗溃疡三联疗法中的潜在应用
目的:我们研究了密缕草(E. speciosa)和MY41g粘土的抗分泌、抑幽门螺杆菌和抗酸性能,并对其在密缕草配制的抗溃疡三联疗法中的潜在应用进行了评价。材料与方法:通过研究Fordtran法,以及温度对pH值的影响,对体外抗酸能力进行评价。研究胃壁内注射0.05 ml(30%)醋酸致慢性胃溃疡的体内活性。在溃疡诱导后的10天内,每天给大鼠服用特殊大肠杆菌和MY41g (ESMY);(ESMY 100+250和200+250 mg/kg)。采用“未愈合”胃溃疡模型:从实验第5天至第18天,大鼠口服ESMY并皮下注射吲哚美辛(1 mg/kg/d)。评估溃疡指数、愈合百分比、粘液分泌、胃酸、组织学、血液学和氧化应激参数。结果:在Fordtran法中,ESMY具有良好的体外中和能力。ESMY治疗能促进慢性胃溃疡的自愈(93.82 ~ 96.14%)。然而,给药吲哚美辛对愈合百分比没有显著影响(90.73-94.60%)。在两种溃疡模型中,当剂量为200/250 mg/kg时,溃疡愈合时黏液质量显著增加(P = 0.001)。ESMY提高抗氧化活性,降低胃酸、脂质过氧化,维持血液系统平衡。结论:ESMY除具有缓冲作用外,其愈合机制还包括降低胃酸、促进粘液生成、胃粘膜再上皮化、改善血液学和抗氧化状态。ESMY可用于传统医学,作为一种治疗胃溃疡的方案。
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