Intraspecific characterization of T. cruzi stocks by the lectins from triticum vulgaris and arachis hypogaea

Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie = International journal of microbiology and hygiene. A, Medical microbiology, infectious... Pub Date : 1984-03-01 DOI:10.1016/S0174-3031(84)80012-8

J. Schottelius , G. Uhlenbruck

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引用次数: 3

Abstract

The agglutination behaviour of four-days-old epimastigote culture forms of T. cruzi, and T. cruzi-like stocks from different South American countries were tested with 15 lectins. Two agglutination types can be distinguished: Typ 1 includes stocks which react with Triticum vulgaris and Aaptos papillota II (WGA-type). Type 2 includes stocks agglutinated by Arachis hypogaea and Aaptos papillata II (PNA-type). The reactions with the lectins from Triticum vulgaris and Aaptos papillata II can be correlated with the presence of N-Acetylneuraminic acid on the cell surface of these flagellates. After treatment with neuraminidase the WGA-type T. cruzi-like Morcego 1354 is agglutinated by Arachis hypogaea but not by the N-Acetylneuraminic acid specific lectins from Triticum vulgaris and Aaptos papillata II.

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利用普通小麦和花生凝集素对黄颡鱼种群进行种内鉴定

用15种凝集素对产自南美不同国家的克氏T. (T. cruzi)和克氏T. (T. cruzi)样砧木的4日龄拟马毛囊培养体的凝集行为进行了试验。可区分两种凝集类型:1型包括与普通小麦和小麦II型(wga型)反应的原料。2型包括由arachhis hypogaea和Aaptos papillata II (pna型)凝集的砧木。这些鞭毛虫与普通小麦和乳突Aaptos papillata II的凝集素的反应可能与这些鞭毛虫细胞表面n -乙酰神经氨酸的存在有关。经神经氨酸酶处理后，wga型T. cruzi-like Morcego 1354可被花生凝集，但不能被普通小麦和乳突Aaptos papillata II的n -乙酰神经氨酸特异性凝集。

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Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie = International journal of microbiology and hygiene. A, Medical microbiology, infectious...

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