Expressions of interleukin-1β,interleukin-6 and tumor necrosis factor alpha in articular cartilage of children with Kashin-Beck disease

Q3 Medicine

中华地方病学杂志 Pub Date : 2014-01-01 DOI:10.3760/CMA.J.ISSN.2095-4255.2014.04.003

Chen Chen, Jinghong Chen, Jun-ling Cao, Wei Wang, Zeng-tie Zhang, Zhan-tian Yang, Bo-quan Yu, Tian-you Ma

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引用次数: 0

Abstract

Objective: To investigate the expressions of interleukin-1β(IL-1β), interleukin-6 (IL-6) and tumor necrosis factor alpha(TNF-α) in cartilage of children with Kashin-Beck disease(KBD) in order to provide a possible mechanism of the disease. Methods: Articular cartilage tissues of 5 KBD children(KBD group) were selected from KBD children autopsy samples keeping in Institute of Endemic Diseases, Medical School of Xi'an Jiaotong University; articular cartilage tissues of 5 normal children(control group) were selected from non-KBD areas of Shaanxi Province, three cases were from accident death children, two cases were the samples of congential malformation of six finger. Expressions of IL-1β, IL-6 and TNF-α in the cartilage were detected using immunohistochemistry; the cells of articular cartilage were divided into three areas (superficial zone, middle zone and deep zone) to analyze the expressions of IL-1β, IL-6 and TNF-α. Results: The expressions of IL-1β in superficial zone, middle zone and deep zone of articular cartilage of KBD group(63.50 ± 7.19, 54.75 ± 5.50, 66.20 ± 9.91) were significandy higher than those of control group (5.75 ±1.26, 0.00 ± 0.00, 0.00 ± 0.00, all P < 0.05). The expression of IL-6 in superficial zone of articular cartilage in KBD group(55.25 ± 6.24) was significandy higher than that of control group (0.00 ± 0.00, P < 0.05). The expressions of TNF-a in all zone of articular cartilage of KBD group(33.25 ± 6.50, 3.75 ± 0.96, 29.80 ± 1.92) were significantly higher than those of control group (3.74 ± 0.82, 0.00 ± 0.00, 0.00 ± 0.00, all P < 0.05), Conclusion: The levels of IL-1β, IL-6 and TNF-α are up-regulated in articular cartilage of KBD children, suggesting that cytokines may play an important role in matrix degradation in KBD children cartilage.

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大骨节病患儿关节软骨组织中白细胞介素-1β、白细胞介素-6及肿瘤坏死因子α的表达

目的:探讨大骨节病(KBD)患儿软骨组织中白细胞介素-1β(IL-1β)、白细胞介素-6 (IL-6)和肿瘤坏死因子α (TNF-α)的表达，探讨其可能的发病机制。方法:从西安交通大学医学院地方病研究所保存的大骨节病患儿解剖标本中选取5例(大骨节病组)的关节软骨组织;选取陕西省非大骨节病地区正常儿童关节软骨组织5例(对照组)，意外死亡儿童3例，先天性六指畸形2例。免疫组化法检测软骨组织中IL-1β、IL-6、TNF-α的表达;将关节软骨细胞分为浅区、中区和深区，分析IL-1β、IL-6和TNF-α的表达。结果:KBD组关节软骨浅、中、深区IL-1β表达量(63.50±7.19,54.75±5.50,66.20±9.91)显著高于对照组(5.75±1.26,0.00±0.00,0.00±0.00，均P < 0.05)。KBD组关节软骨浅区IL-6表达量(55.25±6.24)显著高于对照组(0.00±0.00,P < 0.05)。大骨病组关节软骨各区TNF-a表达量(33.25±6.50,3.75±0.96,29.80±1.92)均显著高于对照组(3.74±0.82,0.00±0.00,0.00±0.00，均P < 0.05)。结论:大骨病患儿关节软骨中IL-1β、IL-6、TNF-α水平上调，提示细胞因子可能在大骨病患儿软骨基质降解中起重要作用。

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来源期刊

中华地方病学杂志我国对人类健康危害特别严重的地方性疾病：克山病、大骨节病、碘缺乏病、地方性氟中毒、地方性砷中毒、鼠疫、布鲁氏菌病、寄生虫、新冠肺炎等疾病，同时还报道多发性自然疫源性疾病。

CiteScore

1.60

自引率

0.00%

发文量

8714

期刊介绍： The Chinese Journal of Endemiology covers predominantly endemic diseases threatening health of the people in the areas affected by the diseases including Keshan disease, Kaschin-Beck Disease, iodine deficiency disorders, endemic fluorosis, endemic arsenism, plague, epidemic hemorrhagic fever, brucellosis, parasite diseases and the diseases related to local natural and socioeconomic conditions; and reports researches in the basic science, etiology, epidemiology, clinical practice, control as well as multidisciplinary studies on the diseases.