PENGARUH KOMBINASI IAA (Indole-3-Acetic Acid) DAN BAP (6-Benzylaminopurine) TERHADAP INISIASI TANAMAN VANILI (Vanilla planifolia Andrews)

Apri Salfiani, Asri Pirade Paserang
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引用次数: 1

Abstract

Provision of Vanilla (Vanilla planifolia Andrews) with traditional cultivation often has problems, including the availability of unhealthy seeds due to disease caused by Fusarium oxysporum. This disease can thwart vanilla plantations up to 85% because the pathogen can infect all parts of the vanilla plant making it difficult to control efforts. Alternative efforts were made to overcome this problem, namely through the initiation process in tissue culture by combining the hormones IAA (indole-3-acetic acid) and BAP (6-benzylaminopurine). This study aims to determine the effect and the maximum concentration of the combination of IAA and BAP on the initiation of vanilla plants. This study was conducted based on a completely randomized design (CRD) with 5 treatments and 3 repetitions. This treatment consisted of: A (MS + 0.1 ppm IAA + 1 ppm BAP), B (MS + 0.2 ppm IAA + 1 ppm BAP), C (MS + 0.3 ppm IAA + 1 ppm BAP), D (MS + 0.4 ppm IAA + 1 ppm BAP), and E (MS + 0.5 ppm IAA + 1 ppm BAP). Observations were made after 40 days from the planting process. The results showed that the concentration of the combination of IAA and BAP can affect the growth and organogenesis of the initiation of vanilla plants (Vanilla planifolia Andrews). Treatment C (MS + 0.3 ppm IAA + 1 ppm BAP) was the best concentration from this study, with average values: number of shoots (1), shoot length (1.73 cm), number of roots (1), and number of leaves (1.33).
传统种植香草(香草planifolia Andrews)往往存在问题,包括由于尖孢镰刀菌引起的疾病而无法获得不健康的种子。这种疾病可以阻碍香草种植园高达85%,因为病原体可以感染香草植物的所有部分,使其难以控制。为了克服这一问题,人们做出了其他的努力,即通过在组织培养中结合激素IAA(吲哚-3-乙酸)和BAP(6-苄基氨基嘌呤)的起始过程。本研究旨在确定IAA和BAP组合对香草植株萌发的影响及其最大浓度。本研究采用完全随机设计(CRD), 5个处理,3个重复。该处理包括:A (MS + 0.1 ppm IAA + 1ppm BAP), B (MS + 0.2 ppm IAA + 1ppm BAP), C (MS + 0.3 ppm IAA + 1ppm BAP), D (MS + 0.4 ppm IAA + 1ppm BAP)和E (MS + 0.5 ppm IAA + 1ppm BAP)。种植40天后进行观察。结果表明,IAA和BAP的组合浓度可以影响香草植物萌发期的生长和器官发生。处理C (MS + 0.3 ppm IAA + 1 ppm BAP)是本研究的最佳处理,其平均值为:芽数(1),芽长(1.73 cm),根数(1),叶数(1.33)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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