Hypolipidemic and Antioxidant Effects of Diallyl Disulphide and Diaceto-Dipropyl Disulphide on Renal Tissue in Alloxan Diabetic Rats - A Comparative Study

Vijay Venkataiah, Divya D, S. H. Puttaswamy, Kashinath R.T
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Abstract

BACKGROUND Diabetic nephropathy (DN) is one of the commonest causes for end stage renal disease. Renal accumulation of lipids is one of the pathological finding seen in diabetic nephropathy. Diallyl disulphide (DADS), a principle component of garlic oil, is known for its hypolipidemic properties. Diaceto-dipropyl disulphide (DADPDS) is a structural analogue of DADS, and is more palatable and less toxic to diabetic rats. Hence this animal experiment study was undertaken to compare the hypolipidemic and antioxidant effects of DADS and DADPDS on diabetic rat renal tissue and to evaluate the better disulphide among the two that may be used as adjuvant drug in treating or preventing diabetic nephropathy. METHODS This was an animal experimental comparative study. 24 male albino rats were grouped (6 rats in each group) into normal, diabetic control, DADS and DADPDS treated diabetic rats. Diabetes was induced in albino rats by intraperitoneal injection of alloxan. DADS and DADPDS were fed by gastric intubation for 90 days. After stipulated time, kidneys were dissected out and its total lipids, cholesterol and phospholipid levels were estimated along with tissue thiobarbituric acid reactive substances (TBARS) levels. RESULTS Renal tissue total lipids, cholesterol and phospholipids were significantly decreased in DADS and moderately decreased in DADPDS treated diabetic kidneys, when compared to diabetic control rats. But TBARS levels were significantly decreased in DADPDS rat kidneys compared to DADS treated rat kidneys. CONCLUSIONS In this comparative study, we note that DADS has better lipid-lowering effect on renal tissue of alloxan diabetic rats compared to DADPDS. On the other hand, DADPDS has low renal toxic effects, as indicated by low TBARS levels and improvement in blood glucose levels, when compared to DADS treated diabetic rats. Hence, DADS can be used as an adjuvant drug, only in atherogenic diabetic patients without nephropathy and DADPDS can be used as an adjuvant drug in diabetic nephropathy patients. KEYWORDS Diallyl-Disulphide, Diallyl-Dipropyl Disulphide, Diabetic Nephropathy, Renal Lipids
二烯丙基二硫醚和二乙酰-二丙基二硫醚对四氧嘧啶糖尿病大鼠肾组织降血脂和抗氧化作用的比较研究
背景:糖尿病肾病(DN)是终末期肾脏疾病最常见的病因之一。肾内脂质积聚是糖尿病肾病的病理表现之一。二烯丙基二硫醚(DADS)是大蒜油的主要成分,以其降血脂特性而闻名。二乙酰-二丙基二硫化物(DADPDS)是一种结构类似于DADS的物质,对糖尿病大鼠更可口,毒性更小。因此,本实验旨在比较DADS和DADPDS对糖尿病大鼠肾组织的降血脂和抗氧化作用,并评价两者中较好的二硫化物可作为治疗或预防糖尿病肾病的辅助药物。方法采用动物实验对比研究。24只雄性白化大鼠(每组6只)分为正常、糖尿病对照组、DADS和DADPDS治疗的糖尿病大鼠。采用腹腔注射四氧嘧啶诱导白化大鼠糖尿病。DADS和DADPDS经胃插管喂养90 d。在规定时间后,解剖肾脏,测定其总脂质、胆固醇和磷脂水平以及组织中硫代巴比妥酸反应物质(TBARS)水平。结果与糖尿病对照大鼠相比,DADS组肾组织总脂、胆固醇和磷脂显著降低,DADPDS组肾组织总脂、胆固醇和磷脂适度降低。但与DADS处理的大鼠肾脏相比,DADPDS大鼠肾脏的TBARS水平显著降低。结论在本对比研究中,我们注意到DADS对四氧嘧啶型糖尿病大鼠肾组织的降脂作用优于DADPDS。另一方面,与DADS治疗的糖尿病大鼠相比,DADPDS具有较低的肾毒性作用,如TBARS水平较低和血糖水平改善所示。因此,DADS仅可作为无肾病的动脉粥样硬化性糖尿病患者的辅助用药,而DADPDS可作为糖尿病肾病患者的辅助用药。关键词:二烯丙基二硫化物,二烯丙基二硫化物,糖尿病肾病,肾脂质
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